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Comparative Transcriptome Analysis Between Somatic Embryos and Zygotic Embryos in Cotton

Author: JinFangYan
Tutor: ZhangXianLong
School: Huazhong Agricultural University
Course: Crop Genetics and Breeding
Keywords: embryo development Gossypium hirsutum somatic embryo zygotic embryo transcriptional profiling stress response gene
CLC: S562
Type: Master's thesis
Year: 2013
Downloads: 6
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Abstract


Cotton is the major textile crop, also as the most important oilseed crop. More recently, the application of plant biotechnology brings cotton breeding to a new field, the Cell regeneration system is a basis for biotechnology applied in breeding, therefore it is significant to research the molecular mechnism of somatic cell regeneration. As a product of somatic cell going through reconstruction under suitable in vitro conditions, the somatic embryo (SE) differentiates into a new plantlet via a zygotic embryogenesis-like process.Here, we present the phenotypic and cellular differences between SEs and zygotic embryos (ZEs) revealed by bistological section scanning using three parallel development stages of the two types of embryos from Gossypium hirsutum cv. YZ1, including the globular, torpedo-shaped and cotyledonal stages. To identify the molecular characteristics of SE development in cotton, the Digital Gene Expression (DGE) system was used to profile the genes active during SE and ZE development. A total of4242differentially expressed genes were identified in at least one developmental stage. Expression pattern and functional classification analysis based on these differentially expressed genes revealed that SE development exhibited remarkable and broad stress responses. RT-PCR for selected stress-related genes with different expression levels further confirmed that the higher expression levels of stress-related genes in SEs than in ZEs. Moreover, stress treatment induced by NaCl and ABA accelerated SE development and increased the transcription of genes related to stress response, in parallel with decelerated proliferation of embryogenic calli under stress treatment. Our data reveal that SE development is the process of plant cells adapting to stress conditions, through genetic adjustment of the balance between cell proliferation and death. These results present a new insight into the molecular mechanisms of SE development and provide strategies that can be used for regulating the developmental processes of somatic embryogenesis.

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CLC: > Agricultural Sciences > Crop > Economic crops > Fiber crops > Cotton
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