Dissertation > Excellent graduate degree dissertation topics show

Cloning, SNPs of Porcine TMOD1, MKK3 and MKK6 Genes and Their Association with Production Traits

Author: WuHui
Tutor: ZhaoShuHong; FanBin
School: Huazhong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: pig clone TMOD1 MKK3 MKK6 productin traits SNPs association analysis
CLC: S828
Type: Master's thesis
Year: 2009
Downloads: 20
Quote: 0
Read: Download Dissertation

Abstract


With the development of the molecular biology theory and technology in the past two decades,a number of molecular markers and candidate genes have been identified and then applied on breeding of animals and plants,which have greatly contributed to the improvement of animal and plant production.Marker assisted selection(MAS) can help researchers and farmers select animals on DNA level and improve the selection accuracy.In the last decade,the selection on swine production traits has made great achievements,owing to the utilization of conventional breeding and molecular breeding approaches.In this study,three important genes including TMOD1,MKK3 and MKK6,which were highly expressed in porcine skeletal muscle,have been investigated as the candidate genes associated with production traits in the pig.The findings of the study are as follows,1) The partial cDNA seqence of porcine TMOD1 gene was obtained.The intron 7 of MKK3 gene and of intron 4 and intron 9 of porcine MKK6 gene were obtained.2) Genetic variants discovery was implemented in the obtained sequences of above three genes.One SNP within exon 7 of TMOD1 gene was identified and this mutation site could be distinguished by MvaI,One SNP within intron 7 of MKK3 gene and two SNPs within intron 4 and intron 9 of MKK6 gene respectively were founded.3) The PCR-RFLP techniques were developed for genotyping of the identified SNPs.Large scale SNP genotyping were carried out in two different pig populations and then the association analyses between the genotypes and the traits were completed.According to the association analysis results from a experimental pig population including Toncheng(n=38),Landrace(n=25),Yorkshire(n=22), Landrace×Yorkshire×Toncheng(LYT)(n=34),Yorkshire×Landrace×Tnonche ng(YLT)(n=38),which was developed by our lab in collaboration with Tnoncheng county,Hubei province.SNP-A134G of porcine TMOD1 gene was significantly associated(p<0.05) with loin pH.SNP-C439T of porcine MKK3 gene is significantly associated with length.SNP-C208T of MKK6 gene is significantly associated with marbling.(4) The association analysis results from the Iowa State University Berkshire×Yorkshire pig resource family showed,SNP-A134G of porcine TMOD1 gene was significantly associated with loin eye muscle area,average back fat, back fat at the lumbar,at 10th rib and at last rib,ham pH and lab loin pH. SNP-C439T of porcine MKK3 gene was significantly associated with loin eye area and firmness.SNP-A324G of porcine MKK6 gene was significantly associated with water holding capactiy and average glycolytic potential. SNP-C208T of MKK6 gene was significantly associated with average daily gain on test and birth weight.In conclusion,TMOD1,MKK3 and MKK6 gene are significantly associated with the production traits in pigs,which may be three important genes affecting production traits in pig.

Related Dissertations

  1. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  2. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  3. Characterization and Identification of Maturity of Pig Manure Composting,S141.4
  4. Polymorphisms in the Promoter Region of Swine BMP7 Gene and Their Association with Reproductive Traits,S828
  5. Studies on Polymorphisms on Exon and Intron of BMP7 Gene and Their Associations with Reproductive Traits in Pigs,S828
  6. Research on Influnce of Silicon and Sulfur on Arsenic Uptake and Accumulation of Rice (Oryza Sativa L.),S511
  7. Study on the Combined Probiotics and Its Effects on Prodution Performance of Pigs,S828.5
  8. Study on Effect of Nutritional Regulation on Pig Production Performance and Discharge of Nitrogen and Phosphorus,S828.5
  9. Cloning Andanalysis of NAC Transcription Factor Gene in Tobacco(Nicotiana Tabacum L.),Q943.2
  10. Cloning and Molecular Analysis of Lovastatin Biosynthesis Related Genes from Monascus Purpureus,TQ927
  11. Research of Resource and Recycling on Pig Excretion in Wenzhou,X713
  12. New Methodology for Mapping Resistance Trait Loci in Crop Cultivar Population,S336
  13. Evaluation on Forage Quality and Biomass Energy Characters of Inbred Vegetative Lines of Napier Grass,S543.9
  14. Characterization of Population Structure and Linkage Disequilibrium of Chinese Soybean Landerace Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  15. Characterization of Population Structure and Linkage Disequilibrium of Chinese Wild Soybean Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  16. Screening Stamen Development Heat-Tolerance Germplasm and Association Analysis of Agronomic Traits with SSR Makers in Upland Cotton,S562
  17. Genetic Diversity, Genetic Variance Association Mapping of Fresh Seed Quality Traits QTLs of G.Max in China,S565.1
  18. The Analysis on Technical Efficiency of Chinese Pig Production,F323.3
  19. Establishment and Application of Triple PCR Assay for Identification of Haemophilus Parasuis, Porcine Pleuropneumonia and StreptococcHS Suis Serotype 2,S858.28
  20. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  21. The Polymorphisms of TLR4 Gene and Function Analysis of C1027A in Suzhong Pig,S828

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Pig
© 2012 www.DissertationTopic.Net  Mobile