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The antihypertensive effect of chlorogenic acid is an important active ingredients of Eucommia, gutta percha is the Eucommia unique hard rubber, both of which are important substances containing Eucommia. Extract purification process cost constraints, the two have not been effective extraction and development, reduce the utilization of Eucommia. Chlorogenic acid and gutta-percha, a very broad market prospects, Eucommia chlorogenic acid and gutta percha extraction and purification process has a very important economic significance and practical value. This topic is different according to the nature of chlorogenic acid and gutta percha dissolved, chlorogenic acid extraction of Eucommia leaf powder extracted through step-by-step approach, first with water, extract purified by NKA-9 macroporous resin and evaporation of the solvent chlorogenic acid; extraction residue after drying with lye extraction decomposition cell wall, and then drying the gutta percha extract gel to give the crude gum extracted with petroleum ether refluxing acetone milky sperm plastic, namely The gutta percha Products. Through the two-step separation of extracted two, help to reduce the cost and improve the utilization of Eucommia. This experiment compared the laboratory spectroscopy of the content of chlorogenic acid detection methods (UV spectrophotometry, paper chromatography - ultraviolet spectrophotometry), chemical methods (complexation colorimetric method, ferric chloride chromogenic method ), high performance liquid chromatography, in terms of precision, stability, repeatability and spiked recoveries were compared, and Eucommia leaf extract chlorogenic acid content were measured paper chromatography - ultraviolet spectrophotometry spectrophotometry and high performance liquid chromatography determination of chlorogenic acid content in the extract accurate and convenient method. Optimized Eucommia chlorogenic acid extraction conditions. Water extraction of chlorogenic acid extraction temperature, extraction time, extraction times, based on the experimental results of the solid-liquid ratio of single factor experiments to optimize the design of response surface has been optimized extraction conditions: extraction time of 2 hours and 40 minutes, extraction temperature of 81 ℃, solid-liquid ratio of 1:21, and chlorogenic acid extraction rate of 0.261%. And alcohol extract optimized results are compared and found water extraction ratio ethanol extraction extraction rate, less impurity content. Study and optimize the macroporous resin NKA-9 purification of chlorogenic acid conditions obtain: water extraction of chlorogenic acid extract directly on the column, the sample concentration 0.13mg/mL so, on the kind of liquid volume with 3 column volume, flow rate 2mL/min, was eluted with 4 column volumes of 10% ethanol solution, chlorogenic acid product purity of 35.8%, a recovery of 85%. To study the the residue extracted gutta percha process conditions, and optimize the alkaline leaching process, optimize conditions for processing 10% NaOH solution at 90 ℃ 3h, processing 1, the solid-liquid ratio of 1:10; optimize oil ether extraction process, optimize the conditions of 85 ° C under hot reflux extraction 2h, solid-liquid ratio 1:16, extraction; determine the analysis glue gel method time of 2h. Expand the experiment using the optimized conditions of the gutta percha Eucommia leaf residue extraction, the extraction rate of 2.431%, a purity of 85.6%.
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