Dissertation > Excellent graduate degree dissertation topics show

The Expression of TCTP in Human Ovarian Cancer and the Study of Effect on Invasion and Metastasis in Ovarian Cancer Cells

Author: ZhouWenJuan
Tutor: WangChangYu
School: Huazhong University of Science and Technology
Course: Obstetrics and Gynaecology
Keywords: ovarian cancer TCTP immunohistochemistry(IHC)siRNA invasion metastasis
CLC: R737.31
Type: Master's thesis
Year: 2012
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


[Objective] To investigate the expression of TCTP in epithelial ovarian cancerand analyze the relationship TCTP and clinical pathological characteristics.[Methods] The expression of TCTP was examined byimmunohistochemistry(IHC) in normal ovarian tissues of18cases, benignovarian tumors of20casses and epithelial ovarian cancerous tissues of60cases.[Results] The immunohistochemical staining shew that the expression ofTCTP in epithelial ovarian cancerous tissues was higher than that in normalovarian tissues and benign ovarian tumors(P<0.05). However, the differencebetween normal ovarian tissues and benign ovarian tumors had nosignificance(P>0.05). The expression of TCTP was associated with clinicalsatge, histological grade and lymph node metastasis, but not age(P>0.05).[Conclusion] TCTP overexpression may promote the progress of malignantphenotype in epithelial ovarian cancer. [Objective] To explore the impact of TCTP in proliferation, invasion andmetastasis in human ovarian cancer cells.[Methods] The expression of TCTP was detected in human ovarian cancercells with Real-time PCR and Western Blot.Synthesized siRNA wastransfected into ovarian cancer cell line with high expression of TCTP, andReal-time PCR and Western Blot was used to detect efficiency of siRNA. Theproliferation of SKOV3change before and after TCTP knockdown weremeasured by CCK-8, the migration and invasion changes were detected bychamber assays.[Results] The expression of TCTP in SKOV3cells was hagher than that inA2780,C13K,OV2008cells.Down-regulated expression of TCTP couldsuppress the proliferation, migration and invasion of SKOV3cells.[Conclusion] Down-regulated of TCTP expression by siRNA could suppressmalignant phenotype in epithelial ovarian cancer.TCTP has the potential tobecome a new therapeutic target for ovarian cancer.

Related Dissertations

  1. The Regulate and Controlof Slug on P53-MDM2 System,R730.2
  2. Effect of Invasive Plants (Conyza Sumatrenss and Alternanthera Philoxeroides) on Soil Carbon and Nitrogen Processes,X173
  3. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  4. Effects on Telomere Length of Epithelial Ovarian Cancer by Chemotherapy,R737.31
  5. The Contribution of the CCl22, CCR4 in the Milky Spots Peritoneal -metastasis of Gastric Cancer,R735.2
  6. Recurrent Ovarian Cancer Surgery Again with Surial Analysis of Relevant Factors,R737.31
  7. Old Ⅲ Colorectal Cancer Survival after Radical Analysis and Evaluation of Chemotherapy,R735.3
  8. Clinical Analysis of Postoperative Recurrent-metastatic Gastrointestinal Stromal Tumors,R735
  9. Surveys of Short-term Efficacy of TP Therapeutic Regimen Applied to 81 Cases of Ovarian Cancer Patients,R737.31
  10. Expressions and Relationships of PI3K/AKT and Apoptosis Related Regulation Factors in Epithelial Ovarian Tumors,R737.31
  11. The Study on Expression of CLIC1 on Lymphatic Metastasis of Mouse Hepatocarcinoma,R735.7
  12. The Location and Expression in Mouse Heptocarcinoma Cell Lines with High and Low Lymphatic Metastatic Potentials.,R735.7
  13. The Study of the Correlation of Prognosis and Pattern Evaluation of Patients with Recurrence of Rectum Cancer,R735.37
  14. α1, 3 fucosyl transferase Ⅳ (Fut4) upregulated increased metastatic potential of squamous cell scc12,R730.2
  15. CXCR1/CXR2 receptor antagonists on mouse ascites hepatoma lymphatic metastatic potential impact,R735.7
  16. Study of Water-Inrush Danger Forecast of Coal Seam Floor Based on Multi Factor’s Fuzzy Clustering Approach,TD745
  17. The Research of Ki67 Expression and DNA Ploidy in Squamous Cell Carcinoma of the Tongue with Different Pattern of Invasion,R739.86
  18. The Expression of the Ratio of VEGF-C/VEGF-D CLIC1 and ECH1 in Gastric Carcinoma and Lymphatic Metastasis,R735.2
  19. The Preliminary Study of Zoledronic Acid on Gastric Cancer SGC-7901 and SGC-7901 / ADR Cell Lines,R735.2
  20. Research on the tort liability of Internet trading platform providers,D923
  21. Anti-tumor Effect of Trastuzumab on Nude Mice Xenografts of Human Ovarian Cancer SKOV3 Cells and Its Synergistic Effect of Action with Docetaxel, Cisplatin,R737.31

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
© 2012 www.DissertationTopic.Net  Mobile