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Betula is widely distributed in southern China, an excellent material for valuable timber species to study the function of genes related to flowering and molecular mechanism for understanding the mechanism of flowering trees is important, but also for regulating luminifera flowering, the election provide a basis for education varieties. Therefore, the study expects flowering pathway through the key regulatory factors LEAFY (LFY), MADS-box and SBP-box gene cloning, expression and function of the preliminary analysis, Betula provide a theoretical basis for molecular breeding. In this study, homology cloning and RACE methods were cloned into luminifera BlLFY, BlMADS1 and BlSPL1 genes. BlLFY length 1305 bp, encoding 321 amino acid homology analysis, BlLFY with chestnut and mango LFY highest homology, up 88%; BlMADS1 transcripts exist two kinds: the first one kind of 1150bp, encoding 254 amino acids, typical MADS-box protein belonging AP1/SQUA subfamily; 2 seed in the 3 'end of the extra 162 bp, resulting in a frameshift mutation encoded MADS-box protein C-terminal deletions, preliminary analysis may be due to intron variable shear caused. Two proteins with homology to the European white birch BpMADS4 were as high as 99% and 95%. BlSPL1 length of 838 bp, the 3 'end miRNA156 binding sites, encoding 134 amino acids, with the European birch BpSPL1 highest homology (99%). Using real-time quantitative PCR method at different developmental stages luminifera male, female flowers, leaves and buds in the gene expression pattern analysis. The results showed that Betula BlLFY gene expression in reproductive organs than vegetative organs, the initial budding male inflorescence inflorescence and high expression; same organs in different developmental stages of expression is different, young and exuberant stage in the developmental expression of is relatively high. BlMADS1 gene expression was not detected in female inflorescence, but in the male inflorescence bud, buds and leaves of the higher expression levels, and with the development of its expression level will decrease. BlSPL1 gene expression in the male inflorescence relatively lower volume, in the initial female inflorescences, buds and leaves of expression is higher. Preliminary analysis BlLFY genes involved luminifera inflorescence and male inflorescence development; BlMADS1 genes may luminifera inflorescence startup related, developmental independent, and may be involved in leaf development; BlSPL1 gene luminifera inflorescence and leaf development related. For further study BlLFY, BlMADS1 BlSPL1 gene function and constructed overexpression of the gene vector and for genetic transformation of Arabidopsis thaliana. At present, access to seed, the ongoing screening and identification of transgenic plants, the function of genes to be analyzed.
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