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Boron is one of the mineral elements necessary for higher plant development. B. napus demand more of boron, the main growing areas of Brassica widespread boron deficiency, application of boron to become the main cultivation of rapeseed production measures. Our group found by screening a large number of Brassica napus germplasm resources, Brassica napus varieties boron efficiency quite different, and is controlled by multiple genes. To provide a basis for more accurate positioning boron efficient gene trait genetic improvement of boron efficient, Brassica napus (Brassica napus) In this study, the research group screening boron efficient cultivars Qingyou 10 (QY10) and boron inefficient cultivar Westar parental hybridization to obtain the F1 generation plants for material, microspore culture establishment of Brassica oilseed rape boron efficient groups (QW DH); SSR (Simple Sequence Repeat) and AFLP (Amplified Fragments the Length Polymorphism) molecular marker construct genetic linkage map; and to of the group and the parents were material, seedling hydroponic pilot survey in two boron levels associated with boron efficiency traits, Boron Efficiency QTL mapping carried out on this basis, mainly to obtain the following results: 1. Brassica napus boron The efficient double haploid population of microspore culture of Brassica napus boron inefficient cultivar Westar, boron efficient cultivars the QY10 and their F1 behalf material, small spores found that the size of the embryo rate by the external environment, culture conditions, and colchicine and other factors, the ultimate test of this building contains 81 lines of Brassica napus boron efficient double haploid population, referred to QWDH. Brassica napus the boron and efficient genetic linkage map QW DH mapping population using SSR and AFLP two molecular markers to construct genetic maps, genetic linkage analysis using JoinMap 4.0 build one containing 652 sites (which molecular genetic linkage map of 20 linkage groups including 478 SSR loci and 174 AFLP loci), the total length of the genetic map of 1785.57 cM, and the average distance of 2.74 cM between markers. 3 the Brassica napus boron efficient analysis of physiological traits (0.25μM B) levels in the low boron, boron inefficient cultivar Westar obvious leaves curl, become darker in color, yellow root, apical black spots and other symptoms, but boron efficient varieties QY10 normal development; efficient parents in the amount of increase in the root length, dry weight, the cumulative amount of boron have shown a distinct advantage. Two boron levels, QW DH group differences transgressive phenomenon. 4 the Brassica napus boron efficient positioning of QTL composite interval mapping software WinQTLCart2.5 14 boron efficiency-related phenotypic data analysis, 38 QTLs were detected in 13 linkage groups: single QTL LOD values ??ranged from in 3.17-11.66 between; single QTL could explain the phenotypic variance contribution rate of between 8.17% -41.26%. From the distribution of QTLs on linkage groups, many sites located in the same linkage group, and overlap among boron efficiency there are pleiotropic QTLs.
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