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Evaluation on the Ecological Safety of Cry1Ab and Cry1Ac Toxin to the Oriental Armyworm, Mythimna Separata and Its Parasitoids

Author: JiangShanJun
Tutor: LuoLiZhi
School: Huazhong Agricultural University
Course: Agricultural Entomology and Pest Control
Keywords: Cry1Ac insecticidal protein Cry1Ab insecticidal protein Armyworm Insecticidal effect Growth and development Multiplication Ability to fly Short foot side Microplitis The umbrella skirt chasing Send flies Parasitic behavior Population Growth
CLC: S482.3
Type: Master's thesis
Year: 2011
Downloads: 30
Quote: 2
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Abstract


For lack of times the target of commercial cultivation of transgenic Bt Transgenic crops migratory pests sticky. Insects Mythimna separata (Walker) (Lepidoptera: Noctuidae) and its parasitoid in light foot side Microplitis Microplitis ecological security assessment research status quo pallidipes (Hymenoptera: Braconidae) and the umbrella skirt chasing Send fly Exorista civilis Rondani (Diptera: Muscidae Send), In this paper, behavior, ecology, physiology, and toxicology methods Cry1Ab and Cry1Ac insecticidal protein armyworm insecticidal effect, the direct effect of growth and development, reproduction, and the ability to fly. At the same time, the study reveals the Cry1Ab insecticidal protein of armyworm parasitoids light foot side Microplitis umbrella skirt chasing Send flies growth and development of the role of transgenic Bt crops, in order to further evaluate the ecological safety of its pests role in the integrated control of new scientific evidence. The main innovation results obtained are as follows: studies clearly Cry1Ac insecticidal protein insecticidal effect against armyworms and growth and development, reproductive effects. The obtained results show that: the feeding of 0 (control), 3, 6, 12, 24, and 48 and 96μg / g CrylAc insecticidal protein artificial diet for 6 d after larval head width, length, and 18 d after weight with the kill the insect protein concentration increased significantly reduced, significantly extend the duration of larval development, mortality was significantly higher. Feeding containing insecticidal protein concentration gt; 12μg / g feed the larvae can not be normal pupation; feeding containing 0,3 6μg / g insecticidal protein feed larvae normally pupate, eclosion pupation With insecticidal the protein concentration increased significantly reduced pupal duration significantly longer; feeding containing 0,3 and eclosion of adult reproductive capacity 6μg / g toxic protein feed larvae were significantly different, spawning the early rise with the insecticidal protein concentration significantly prolonged, significant reduction in fecundity. These results suggest that growth and development, reproduction Cry1Ac insecticidal protein armyworm has a significant impact. Clear insecticidal effect Cry1Ab insecticidal protein armyworm instars clarify Cry1Ab insecticidal proteins cause larval food consumption, and food use efficiency is the major cause of death. Cry1Ab insecticidal protein LC50 values ??of 1-6 larvae in the study clearly identified Cry1Ab insecticidal protein insecticidal effect on the young larvae poor 4-6 instar larvae insecticidal effect at the same time, the application gravimetric determination of Cry1Ab insecticidal protein on the armyworm six larvae during development of food and food consumption, fecal excretion, body weight gain, approximate digestibility (AD), the crude growth efficiency (ECI) and pure growth efficiency (ECD) effect. The results show that: feeding 0 (control), 12.5, 25, 50, 100 and 200μg / g CrylAb insecticidal protein artificial feed larvae correction food intake and fecal excretion, the amount of weight gain with increasing concentration of insecticidal protein significantly reduced. ECI and ECD of larvae feeding insecticidal proteins are significantly lower than the control group, larvae, and significantly decreased with increasing the insecticidal protein concentration, feeding containing the insecticidal protein concentration gt; 25μg / g feed larvae ECI and ECD is negative, and the greater the concentration of ECI and ECD lower. The AD value but the AD with the insecticidal protein concentration increased significantly elevated and feeding insecticidal proteins larvae were significantly higher than the control larvae. These results suggest that Cry1Ab insecticidal protein of six instar armyworm larvae mechanism by to inhibit larval food consumption, reducing food digestibility and utilization of the larvae, thus inhibiting the growth and development of the larvae, and eventually death. To clarify the mechanism of Cry1Ab insecticidal protein armyworm larvae lethal effect. The study reveals the Cry1Ac insecticidal protein sublethal concentrations of armyworm growth and development, reproduction, and the ability to fly. Armyworm larvae fed on artificial diet containing sublethal concentrations of Cry1Ac insecticidal protein, weight as the protein concentration increased significantly reduced larval development significantly extend the duration; with a Cry1Ac insecticidal protein concentration increased, pupal weight significantly reduced pupal period was significantly extended; adult fresh weight, dry weight, the weight of the flight muscles with protein concentration were increased significantly reduced; eclosion of adult feeding Cry1Ac insecticidal protein preoviposition significantly longer than the control, but between the concentration There was no significant difference. Fecundity, mating frequency, spawning duration with elevated protein concentration was no significant change; 1-day-old adults the ability to fly (flight distance, time and speed) with Cry1Ac insecticidal protein concentration increased significantly reduce. These results suggest that the growth and development of sublethal concentrations of Cry1Ac insecticidal protein armyworm, the flight has a significant inhibitory effect, but has little effect on adult reproductive. The study evaluated the effect of Cry1Ab insecticidal protein on the growth and development of short foot side Microplitis. Parasites in continuous feeding containing short Cry1Ab insecticidal protein 1,2,4 and 8μg/g4 of of kinds of dose armyworm foot side ditch Braconidae growth and development of observation results show that the feeding containing the Cry1Ab insecticidal protein from sticking insects as the host of the short foot side ditch Braconidae egg - larvae development calendar period to control armyworms as host of extend pupa (cocoon) weight and adult weight significantly with the decline, but the pupal duration and adult life did not significantly with impact . In addition, the the short foot side Microplitis development calendar period as host feeding Cry1Ab insecticidal protein dose increase significantly extended cocoon weight and adult body weight decreased significantly with the increase in the dose of insecticidal protein, adult longevity downward trend, but the armyworm feeding these doses insecticidal protein of short foot side Microplitis pupal without significant impact. These results suggest that the Cry1Ab protein has a significant impact to the short foot side Microplitis the growth and development, and the higher the concentration, the impact of the more significant role. The study evaluated the effects of Cry1Ab insecticidal protein sheds chase parasitic behavior and population growth Send flies, revealed for the first time Cry1Ab insecticidal protein can promote population growth of parasitic flies, there are obvious synergies in the control of armyworm . Compared with the control the umbrella skirt chasing Send flies feeding on Cry1Ab insecticidal protein 6.25 and 25μg / g doses armyworm parasitism rate and average spawning no significant differences in volume, indicating that Cry1Ab insecticidal protein sheds chase Send flies no significant impact on the host selection behavior. In addition, in the feeding 3.13μg / g of Cry1Ab insecticidal protein armyworm development the umbrella skirt chasing Send flies survive (the flies) rate of 40%, significantly higher than for the host to control armyworms survival rate (6.7%) , and dose rate of parasitic feeding 6.25 (3.3%) and 12.5μg / g (3.3%). And the the parasitic flies survival difference between the control, 6.25 and 12.5μg / g dose was not significant. These results suggest that a low concentration (3.13μg / g) Cry1Ab insecticidal protein can significantly increase the umbrella the skirt chasing Send flies survival. Further results also showed that, with increasing the insecticidal protein concentration the host larvae mortality was significantly increased, the moth was significantly lower. The as host armyworm mortality to feeding 3.13μg/gCry1Ab insecticidal protein is only 14%, while feeding 12.5μg / g Cry1Ab mortality up to 53%. These results suggest that Cry1Ab insecticidal protein and parasitic flies in the control of a host of pests has obvious synergies to provide new ideas, and thus can solve pest resistance to Bt transgenic crops.

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CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Pesticides
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