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Nucleic Acid Binding Behaviors and Cytotoicity Properties of Metal Polypyridyl Complex

Author: ZhanLei
Tutor: TanLiFeng
School: Xiangtan University
Course: Inorganic Chemistry
Keywords: Nucleic acid Complexes Interaction The anti-tumor activity
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 57
Quote: 0
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Abstract


Nucleic acid biological macromolecules by many nucleotide polymerization compound, is one of the most basic substance of life. The nucleic acid is not only the basic genetic material, and also occupy a prominent position in the protein biosynthesis, and thus play a decisive role in a series of major life phenomena of growth, genetic mutation. Therefore, the study of nucleic acid structure and function, can help people learn more about the nature of the phenomenon of life, from the molecular level to understand the pathogenesis of certain diseases. This article provides an overview of the structure of nucleic acids, compounds with DNA research progress, and experimental research complexes with nucleic acid mechanism. The main work is as follows: The first chapter is the preamble of the nucleic acid composition of its various structures, respectively, of the metal complexes with DNA interaction mode anti-tumor activity, research methods, research progress, as well as different metal complexes were reviewed. Chapter II design and synthesis of metal ruthenium complexes [Ru (IP) 2 (tbtc)] 2, using electronic absorption spectroscopy, fluorescence spectroscopy and EB competitive binding experiments and viscosity method studied by means of [Ru (IP) 2 (tbtc) between 2 and calf thymus DNA and yeast RNA interaction mechanism. With the increase of the concentration of DNA or RNA, with the substance absorption peak appears in the ultraviolet and visible region of a few of the more obvious hypochromic effect was found by experiment. The fluorescence intensity of the EB-DNA and EB-RNA with [Ru (IP) 2 (tbtc)] 2 concentration increases more and more weak fluorescence quenching effect. Are inserted into the role of the mode of action of the complexes with DNA and RNA, and complexes of [Ru (IP) 2 (tbtc)] 2 interaction with DNA is stronger than the interaction with RNA. The complexes on human leukemia cell line (HL-60) showed good anti-tumor activity. Chapter III design and synthesis of metal complexes of cobalt [Co (Phen) 2 (HPIP)] 3, the same spectroscopy and viscosity method to study the complex [Co (Phen) 2 (HPIP)] 3 with calf thymus DNA and the interaction mechanism between the yeast RNA. It was found that the complexes with the increase of the concentration of DNA or RNA, an absorption peak in the ultraviolet and visible region of several hypochromic effect is not very obvious. The complex in solution with a strong fluorescence, and fluorescence was significantly enhanced after adding DNA or RNA. The complexes [Ru (IP) 2 (TBTC)] 2 interactions with RNA stronger than the interaction with DNA. The complexes on human leukemia cell line (HL-60) showed good anti-tumor activity. Chapter design and synthesis of metal copper complexes [Cu (NMIP) 2] 2, uses the same method of spectroscopy and viscosity studies of the compound with calf thymus DNA and yeast RNA mode of action, and by spectroscopic methods the size of the compound with DNA and RNA intensity. The complex human hepatoma cells (HepG) and human leukemia cell line (HL-60) did not show very good anti-tumor activity. The fifth chapter, the main content of the paper is summarized and further outlook of future work.

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