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Objective: Platelet-rich fibrin is a new generation of blood concentrate, rich in a variety of growth factors. In this study, by the establishment of sheep mandibular defect model, Prosthodontics film group and blank group as a control, to explore the role of PRF in the repair of bone defects, and provide reference for clinical research and application. Methods: 8-12 months old healthy male goats 3 3 5 mm in diameter were prepared using the autologous control methods in the sheep mandibular buccal side of the front teeth area, deep 2mm circular bone defects. 3 defects ABC is divided into three groups: A group placed PRF membrane as the experimental group B group coverage Haiao the repair film as a standard control group, C group does not place any material as a blank control group. Turn in 4 weeks, 8 weeks and 12 weeks, the animals were sacrificed to obtain specimens, specimens observed X-ray image observation, HE slice observation, comparing three groups of osteogenic effect and pathological picture color HPIAS-2000H high-definition multimedia text analysis software bone histomorphometry analysis, calculate the percentage of new bone trabecular area. SPSS 13.0 statistical software for statistical analysis. The results: 1. General observation: no animal deaths in the experimental surgery area healed well, No wound infection occurred, the normal color of soft tissue morphology. Four weeks, the control group did not completely healed, the experimental group and the standard control group has almost healed. 8 weeks and 12 weeks, three groups of bone defects have been covered, the difference was not significant. The 2.X Line observation: 4 weeks blank control group visible more visible circular image transmission shadow, the experimental group and the standard control group bone defect transmittance shadow a lesser extent, the boundary is not clear; 8 weeks, the experimental group, bone and standard control group transmission Shadow obvious defect; the transmissive shadow of the blank control group slightly. 12 Wednesday group defect showed no obvious transmission shadow. 3.HE slice microscope: the rare four weeks blank control group, the new bone trabeculae, and a smaller number of osteoblasts, more fiber content; experimental group and standard control new bone trabeculae relatively more surface osteoid deposition , osteoblasts active, arranged in a row, the fiber component is less. 8 weeks experimental group and the standard-control group, trabecular bone thickening increased, interconnected mesh osteoblasts; less fiber content; blank control group, trabecular bone is relatively slender, more fiber content. 12 weeks experimental group and the standard control group visible coarse obvious lamellar structure of trabecular bone, blank control group, no lamellar structure. Histomorphometry analysis: the experimental group at 4 weeks, 8 weeks, 12 weeks, new bone trabecular area percentage (35.92 ± 3.89,60.72 ± 4.53,74.73 ± 3.64) than the control group (22.56 ± 4.59, 52.18 ± 5.31,66.77 ± 4.52), the difference was statistically significant (P lt; 0.05). Experimental group and the standard control group at 4 weeks, 8 weeks, 12 weeks, new bone trabecular area percentage (34.36 ± 3.61,61.95 ± 5.24,76.24 ± 3.35) were not significantly different (P gt; 0.05). Conclusion: Platelet-rich fibrin in the repair of bone defects significantly promote bone. Platelet-rich fibrin in the smaller range of bone defects independent defects as a bone graft material.
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