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Effect of Polysaccharides from Pleurotus Ferulae on Immune System in Mutine Cervical Cancer U14

Author: YangLin
Tutor: GongPing
School: Shihezi University
Course: Immunology
Keywords: PFP murine uterocervical carcinoma number14 (U14) immunologic function
CLC: R737.33
Type: Master's thesis
Year: 2011
Downloads: 61
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Abstract


Objective:To investigate polysaccharides from Pleurotus ferulae (PFP) for their regulative effects on Immune System and murine uterocervical carcinoma number14(U14).Methods:Animal tumor models have been established, then ramdomly divided them into 4 groups. Model group, DDP group, PFP group and PFP+ DDP group, given various treatment for 10 days; After treatment, the weight of the tumor was observed to evaluate the growth inhibitory effect of PFP. The effect of PFP on the proliferation and transformation of lymphocytes in spleen was determined by MTT assay, the effect of PFP on the killing activity of NK cell was determined by lactate dehydrogenase releasing assay. Mouse peritoneal macrophages phagocytosis of chicken red blood cell (semi-in vivo method) test. The tumor weight was measured after treatment. The expression of TNF-a and IFN-y protein in Peripheral blood were assayed by ELISA. The blood T lymphocyte subsets were assayed by using immunofluorescence staining and flow cytometry. Continuous variables were presented as mean±d and difference between two groups were tested using t-test.Results:To explore the cell immunologic function of PFP on rumor-bearing mice.1. PFP can increase the tumor-bearing mouse peritoneal Mφphagocytosis, phagocytic percentage and phagocytic index were higher than the model control group, the difference was statistically significant (p<0.001), and tumor-bearing mice with cisplatin phagocytic percentage and phagocytic index compared with the control group decreased, the two drugs combined group tumor phagocytosis of murine peritoneal Mφthan cisplatin group (p<0.001).2.PFP promoted the proliferation of lymphocytes, the killing activity of NK cell and the function of macrophage, significantly difference (p<0.001). PFP could improve immunosuppression by DDP.3. The polysaccharide from Pleurotus tumor-bearing mice treated T, B lymphocytes ConA, LPS proliferation activity are different degrees of increased (P<0.5), while explosed by cisplatin, the T, B lymphocytes was lower than PFP (P<0.01), the two drugs in combination T, B lymphocyte activity when compared with cisplatin alone slightly, but not statistically significant.4.The ratio of CD4+/CD8+of blood T lymphocyte subsets was increased in the tumor saline control group, especially in tumor the DDP control group3. But the ratio of united the two drugs got close to the normal group.To explore the molecule immunologic function of PFP on tumor-bearing mice. It observed that the content of TNF-a、IFN-y of the model group mice was obviously under the natural level and the level of PFP group was significantly higher than model group. Inhibition rate:The tumor weight of model group’s mice was heavier than DDP group, PFP group and PFP +DDP group, significantly difference (p<0.01). Tumor weight of the two drugs in combination group compared with cisplatin to reduce, significantly difference (p<0.01). The tumor inhibition rate was 39%, 46.5%,65.1%.Conclusion:1.PFP promoted the proliferation of lymphocytes, the killing activity of NK cell and the function of macrophage. PFP could improve immunosuppression by DDP.2. Tumor regression exposed in PFP more effectively than untreated mice. The expression level of TNF-a and IFN-y was remarkably increased.3. The tumor inhibition rate was 39% after exposed by PFP. The rate was increased to 65.1% after exposed by PFP united the DDP. It indicated that PFP can raise the function of anti-tumor activity.4. The ratio of CD4+/CD8+of blood T lymphocyte of united the two drugs got close to the normal group. It indicated that PFP can improve the immune function of tumor-bearing mice, the pathogenesy which was unknown need to search.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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