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Background entering the 21st century, with the rapid development of the global economy, the human disease spectrum corresponding changes in malignant threat to human health is increasingly prominent. Currently, pancreatic malignancy in 4 fatal disease, its incidence has increased year by year. As the blood and lymph node metastasis in pancreatic cancer earlier, resulting in difficulties in early diagnosis, the mortality rate is high, the median survival time after diagnosis of pancreatic cancer patients are not more than six months, the 5-year survival rate is less than 5%. Therefore, in-depth study molecular biological mechanisms of invasion and metastasis of pancreatic cancer, will play an important role to improve the diagnosis and treatment of pancreatic cancer. In recent years, with the deepening of research in molecular biology, the mechanism of tumor metastasis gradually been recognized, the study found that tumor metastasis involves at least two types of genes, gene transfer that promote metastasis suppressor gene. To treat cancer by inhibiting tumor metastasis can be considered, and will become a new way for the treatment of cancer. Breast cancer metastasis suppressor gene 1 (breast-cancer metastasis suppressor 1, BRMS1) recently reported abroad tumor metastasis suppressor gene BRMS1 metastasis inhibition of pancreatic cancer research has potential applications. BRMS1 gene does not affect the growth of the primary tumor, but can inhibit tumor cell metastasis to distant sites, as a newly discovered tumor metastasis suppressor gene, has a good prospect of application for the treatment of pancreatic cancer. Objective To establish an effective model of the human nude mice xenograft model of pancreatic cancer, study the BRMS1 gene on human pancreatic cancer cells the ability of tumor metastasis in nude mice, looking for the gene therapy of pancreatic cancer. Recombinant vector pEGFP-C1-BRMS1 liposomal transfection technology will be built, pEGFP-C1 empty vector stably transfected into human pancreatic cancer cell line PANC-1 group inoculated three groups (transfected with empty vector group subcutaneous the untransfected group) cells in nude mice after five weeks, the mice were sacrificed, and the tumor, lymph nodes and lung tissue for histopathological examination. Results pEGFP-C1-BRMS1 recombinant vector, pEGFP-C1 empty vector transfected into human pancreatic cancer cell line PANC-1, and the establishment of a human pancreatic cancer transplanted into nude mice model, no significant difference between the three groups of nude mice tumor size, However, groups of nude mice transfected with regional lymph node metastasis was significantly lower than the empty vector group and untransfected group. Conclusion and the BRMS1 gene does not affect the growth of human pancreatic cancer cells transplanted into nude mice, but inhibition of regional lymph node metastasis of human pancreatic cancer cells transplanted into nude mice.
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