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Objective: To observe the plasma colloid osmotic pressure (COP) on cerebral ischemia / reperfusion (Ischemia / Reperfusion, I / R) cerebral edema (Cerebral Edema), intracranial pressure (ICP); further observation of brain water content rate of neuronal apoptosis, whether for the clinical application of colloidal provide basic theoretical basis for the treatment of cerebral edema and intracranial hypertension. Materials and methods: 32 clean healthy adult male SD rats were randomly divided into four groups: cerebral ischemia in 10% human serum albumin group (serum albumin values), cerebral ischemia hydroxyethyl starch 130/0.4 sodium chloride (Hes) cerebral ischemia model group (Control) and sham-operated group (Sham). 2h pull out the line in the right middle cerebral artery occlusion (middle cerebral artery occlusion, MCAO) bolt middle cerebral artery reperfusion (I / R) for the treatment of the starting point, cerebral ischemia and 10% human serum albumin group, cerebral ischemia hydroxyl ethyl starch 130/0.4 sodium chloride group were used syringe pump via the tail vein injection of 10% human serum albumin (80mg * kg-1 * d-1) and hydroxyethyl starch 130/0.4 sodium chloride (206 ml * kg-1 * d-1); sham group and cerebral ischemia model group has not interfered with. OUTCOME MEASURES: 0,2,6,12,18,24 h after surgery were used Okometer BMT-923 colloid osmotic pressure measuring instrument to measure the plasma colloid osmotic pressure (COP) Integraneuro sciences Camino (?) MPM-cranial internal pressure monitor monitoring the rat intracranial pressure (ICP) changes. The rats were sacrificed after 24h of treatment, the fast decapitation remove the right cerebral hemisphere (the ischemia side of the) were randomly divided into two groups, and a group of wet and dry weight measurement of brain water content; another group to take the the lesion surrounding tissue frozen sections, using The double immunofluorescence law observe neuronal apoptosis. Data Analysis and Statistical analysis: All data is processed by the SPSS 13.0 statistical software, according to data of different types of statistical processing. Measurement data follow a normal distribution (mean ± standard deviation) (x ± S) said; groups were compared using one-way ANOVA analysis of variance. Homogeneity of variance between groups for multiple comparisons using LSD analysis; unequal variances between the two groups using Dunnett's T3 multiple comparisons; P lt; 0.05 difference was statistically significant. Results: 1. Changes in plasma colloid osmotic pressure (COP): surgery each group showed no statistically significant difference in the COP, COP was no significant change in the model group, sham group (all P gt; 0.05), prompted not give colloidal liquid animal model COP remained stable; albumin group over time, hydroxyethyl starch group COP group compared with statistical differences (all P lt; 0.05), suggesting that input of colloid albumin group, hydroxyethyl starch group COP have significantly increased; between albumin and hydroxyethyl starch group, albumin and model groups, albumin and sham group, hydroxyethyl starch with the model group, hydroxyethyl starch group and the sham group COP group all P lt; 0.05, suggesting that the albumin group, hydroxyethyl starch group using colloidal liquid than that the application of colloidal solution model group, sham group significantly increased COP. 2h after and beyond the albumin group at each time point, hydroxyethyl starch group COP (mmHg, 1mmHg = 0.133kPa) are significantly higher than the model group and the sham group (all P lt; 0.05), and the albumin group hydroxyethyl starch group COP differences (P lt; 0.05), reached the peak (COP = 19.20mmHg), 2h albumin group COP after surgery, peaked at 6h after surgery hydroxyethyl starch group COP (COP = 13.488mmHg ). Gradually decline after 24h after albumin, hydroxyethyl starch group COP is still higher than the model group and sham operation group, model group and the sham group COP time point showed no differences (P gt; 0.05). Changes in intracranial pressure (ICP): The the postoperative albumin group and the hydroxyethyl starch group postoperative ICP with the model group and the sham group were not statistically different set of ICP within the normal range; sham operation group ICP change group within 24 hours showed no significant difference (P gt; 0.05) suggesting that the sham group without brain damage so the ICP has been in a steady state; the postoperative albumin group, hydroxyethyl starch and model groups over time ICP group were statistically differences (all P lt; 0.05) prompted MCAO surgery caused brain damage leading to protein group, hydroxyethyl starch and model groups increased intracranial pressure (ICP) and reached a peak in 24 hours; surgery after 2h albumin group, and model groups of hydroxyethyl starch ICP (mmHg, l mmHg = 0.133kPa) that significantly the (albumin group ICP = 11.31mmHg higher than the sham group the the Hydroxyethyl starch group ICP2h = 12.5mmHg model group ICP2h = 11.500 mmHg the sham group ICP2h = 7.950mmHg P lt; 0.05), with the time in addition to the sham group other groups the ICP are gradually elevated to 24 h, reached the peak (albumin group ICP = mm22 .35 Hg, the hydroxyethyl starch group ICP = 22.763mmHg model group ICP24h = 23.325mmHg than fake surgery group ICP = 8.150mmHg, all P lt; 0.05); albumin group, but each time point hydroxyethyl starch group ICP There was no difference in the model group (all P gt; 0.05), albumin and hydroxyethyl starch group ICP no difference (all P gt; 0.05). Points of the sham group showed no significant difference in ICP. Group of albumin, hydroxyethyl starch group, model group ICP group showed no significant difference (P gt; 0.05), but significant difference (P lt; 0.05) compared to the sham group prompted the albumin group, hydroxyethyl based starch group, model group, no significant differences due to MCAO lead to cerebral edema and intracranial pressure level did not decline due to the application of colloidal solution. Groups ischemic hemisphere brain moisture: 24h after albumin group, hydroxyethyl starch group and model group brain tissue water content was significantly higher than the sham group (F = 6.326, P = 0.008), which White proteomics BWC of = 80.457% hydroxyethyl starch group BWC of = 80.161%, the model the the group BWC of = 80.589%, sham group BWC of = 78.72%, all P lt; 0.05, but further multiple comparisons using LSD method row group found group of albumin and hydroxyethyl starch, albumin, model group, hydroxyethyl starch with model group, brain moisture content (BWC) was no significant difference (P = 0.552, P = 0.790, P = 0.394), were P gt; 0.05. 4. ischemic lesions brain tissue around the death of the neurons tune: 24h after the albumin group, and model groups of hydroxyethyl starch brain yuan apoptosis rate significantly higher than that in the sham group (F = 44.835, P = 0.000), the albumin group = 44.252% = 44.271% hydroxyethyl starch group, model group = 42.817%, sham group = 3.256%, all P lt; 0.05) further by LSD the law row group multiple comparisons between albumin and hydroxyethyl starch group, the apoptosis rate of albumin with the model group, hydroxyethyl starch with the model group of neurons was no significant difference (P = 0.997, P = 0.743, P = 0.740), all P gt; 0.05 prompt application of colloidal liquid neuronal apoptosis did not significantly improve; albumin group and the sham group, hydroxyethyl starch group and the sham group, model group and sham neuronal apoptosis rate of the surgery group (P = 0.000, P = 0.000, P = 0.000), all P lt; 0.05, with a statistically significant difference. . Conclusion: Through this experiment, we observed: both natural colloidal - albumin, or synthetic colloid - hydroxyethyl starch can effectively improve the plasma colloid osmotic pressure, but as the plasma colloid osmotic pressure increase did not observed intracranial pressure lowering and cerebral edema. Increase plasma colloid osmotic pressure did not improve neuronal apoptosis. 3. Requires more in-depth study of the existence of a dose-effect relationship, the safe range colloid osmotic pressure regulation, regulation of plasma colloid osmotic pressure whether to reduce intracranial pressure to reduce brain edema and other issues between the colloid osmotic pressure and intracranial pressure.
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