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Objective measurement and comparison adipokine apelin and chemerin impaired glucose tolerance in normal pregnant women, the changes in the level in the serum of pregnant women and pregnant women with gestational diabetes, to analysis of the relationship between adipokine apelin and chemerin and other related metabolic and clinical biochemical indicators, explore fat factor apelin and chemerin possible role in insulin resistance during pregnancy and gestational diabetes pathogenesis. Methods pregnant women screened for gestational diabetes at 24-28 weeks pregnant line after 50 grams of glucose powder load screening, oral 75g glucose tolerance test (OGTT) check abnormal results, according to the different venous blood glucose values ??at each time point pregnant women were divided into three groups: normal glucose tolerance group (NGT), 26 cases of abnormal glucose tolerance group (GIGT) 20 cases of gestational diabetes group (GDM) in 28 cases. According to the index value of pregnancy weight pregnant women were divided into two groups: normal weight group of 34 patients, 40 cases of overweight. All pregnant women to pre-pregnancy weight and family economic conditions survey. Measuring height (height), weight (weight), diastolic blood pressure (DBP) and systolic blood pressure (SBP), calculation of body mass index (BMI) = weight (kg) / height (m2); venous blood taken after fasting and 75g glucose load fasting serum adipokine apelin and chemerin double antibody sandwich enzyme-linked immunosorbent assay (ELISA), two hours after a 75g glucose load apelin and chemerin; simultaneous determination of all subjects triglyceride (TG), total cholesterol (TC ), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG), fasting serum insulin (FINS), two hours after 75g glucose load glucose (2hBG), 75g glucose 2 hours postload insulin (2hPINS); homeostasis model assessment insulin resistance index (HOMA-IR), HOMA-IR = FBG × FINS/22.5; the application SPSSll.5 statistical software for each set of data comparison and correlation Analysis. Results (1) two hours after the fasting and 75g glucose load serum apelin content in the NGT group GIGT group and GDM group gradually increased [NGT: 392 ± 17pg/mL,, 452 ± 21pg/mL; GIGT: 466 ± 29pg/mL , 571 ± 39pg/mL; GDM: 468 ± 31pg/mL, 575 ± 40pg/mL. Serum apelin content GIGT group and GDM group than NGT group, and the difference was statistically significant (P lt; 0.05); GIGT and GDM group comparison, the difference was not statistically significance (P gt; 0.05)]; fasting and serum two hours after the glucose load apelin content in overweight was significantly higher than the normal weight group [overweight: 479 ± 42pg/mL, 582 ± 39pg/mL; normal weight group: 397 ± 31pg/mL, 458 ± 33pg/mL The difference was statistically significant (P lt; 0.05)]. (2) fasting and 2 hours after glucose load serum chemerin content in the NGT group, GIGT group and GDM group gradually increased [NGT: 58.8 ± 19.2ug / L 66.8 ± 21.2ug / L; GIGT: 62.8 ± 18.2ug / L, 69.9 ± 22.1ug / L; GDM: 65.2 ± 17.6ug / L, 72.8 ± 23.1ug / L, but the differences among the groups had no statistical significance (P gt; 0.05)]; fasting and 2 hours after glucose load serum in chemerin content of overweight was significantly higher than the normal weight group [overweight: 73.5 ± 9.2ug / L 76.9 ± 22.3ug / L; normal weight group: 59.4 ± 9.1ug / L, the 65.9 ± 9.3ug / L. The difference was statistically significant (P lt; 0.05)]. (3) two hours after the fasting and 75g glucose load serum insulin content in three groups of pregnant women [NGT: of 14.16 ± 2.42uIU / L, 36.52 ± 21.23uIU / L; GIGT: of 21.86 ± 3.87uIU / L, 57.23 ± 39.58uIU / L; the GDM: 19.08 Soil 3.65uIU / L, of 45.14 ± 30.56uIU / L. Pairwise comparisons among the three groups, the differences were statistically significant (P lt; 0.05)]. (4) reflects the insulin sensitivity index, insulin resistance index (HOMA-IR) in three groups of pregnant women in NGT, GIGT, GDM, respectively [2.85 ± 0.48,3.71 ± 0.92,3.98 ± 0.76, NGT group than in the other two groups, the difference was statistically significant (P lt; 0.05), while GIGT and GDM, the difference was no statistical significance (P gt; 0.05)]. (5) Pearson linear correlation analysis showed: fasting plasma apelin levels and HOMA-IR during pregnancy BMI, TC, LDL-C, of ??FBG, of FINS, chemerin showed a positive correlation (r = 0.58, P lt; 0.01; r = 0.47, P lt ; 0.01; r = 0.35, P lt; 0.01; r = 0.34, P lt; 0.01; r = 0.32, P lt; 0.05; r = 0.26, P lt; 0.05; r = 0.35, P lt; 0.05). Fasting Chemerin level with pregnancy BMI, HOMA-IR, TG, LDL-C, FBG, FINS, 2hBG, 2hPINS was positive related (r = 0.68, P lt; 0.01; r = 0.49, P lt; 0.01; r = 0.46, P lt; 0.01; r = 0.37, P lt; 0.05; r = 0.32, P lt; 0.05; r = 0.26, P lt; 0.05; r = 0.19, P lt; 0.05; r = 0.22, P lt; 0.05). (6) Multiple linear regression analysis showed that: HOMA-IR, pregnancy BMI are the impact of of serum apelin the independent relevant factors. Pregnancy BMI, HOMA-IR, respectively, is the impact of of serum chemerin the independent relevant factors. Conclusions (1) gestational diabetes serum adipokine apelin and chemerin levels and the apelin and chemerin and insulin resistance in the development of close relations. (2) Apelin, chemerin, HOMA-IR was positively correlated in the sera of pregnant women. (3) Apelin and chemerin In the IR occurrence development by changing the insulin resistance index, lower serum apelin and chemerin levels in the future may become a new target for treatment IR.
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