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Construction and Evaluation of Channel Catfish Virus (CCV) ORF6 Gene Suicide DNA Vaccine

Author: XiongHaiLin
Tutor: WangWeiMin；LiuXueQin
School: Huazhong Agricultural University
Course: Aquaculture
Keywords: Channel catfish virus (CCV) ORF6 gene Suicidal DNA vaccine Building Immune effect
CLC: R392.1
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


Of this study was to construct a the Ictalurus traditional DNA vaccine and \1. Spotted fork tail channel catfish virus (Channel Catfish Virus, CCV) \DNA vaccine vector the pSFV with conventional DNA vaccine vector pcDNA3.1, transforming E. coli DH5α, the plasmid was extracted to construct a \performing PCR amplification, extraction methods such as plasmid digestion and sequencing confirmed the recombinant plasmid was constructed correctly. 2 CCV the ORF6 gene expression in the cells will be verified \are expressed in the cells, but the expression of \3.CCV \control of three additional immune agent (I \0 week, two weeks, four weeks, six weeks, 8 weeks at different times such as blood testing blood phagocytic activity, serum lysozyme activity, blood total protein content indicators. Experimental results and analysis are as follows: (1) CCV suicide DNA vaccine induced leukocyte phagocytic activity than the control group there was significant difference (P lt; 0.05) were higher than the other groups. Show that CCV \The experimental results show that the influence The Channel Catfish phagocytic activity of many factors, experimental groups has been strengthened. (2) Ⅰ, Ⅱ, Ⅲ group IV serum lysozyme content higher than the group Ⅴ group Ⅲ and group Ⅳ was significantly higher than the other groups (P lt; 0.05), Ⅲ group was significantly higher than that in group Ⅳ (P lt; 0.05). Show that the the CCV \(3) in each experimental group can cause channel catfish serum protein content increased, but from the group there were significant differences of view, CCV \and immunological reagents the cross joint action. 4.CCV \Statistics after immunization groups Ictalurus growth, found each group growth well, the difference between the groups was not significant, indicating that the group of immune agent Growth of Channel Catfish.

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CLC: > Medicine, health > Basic Medical > Medical Immunology > Immunobiology
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