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The Establishment and Mechanism of Ovalbumin Immune Tolerance in DO11.10 Mice

Author: ZhaoYanQing
Tutor: ChenDeKun
School: Northwest University of Science and Technology
Course: Bioinformatics
Keywords: DO11.10 mice Ovalbumin Peripheral immune tolerance
CLC: R392.9
Type: Master's thesis
Year: 2011
Downloads: 35
Quote: 0
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Abstract


Immune tolerance to the immune system without antigen-specific immune response or low response phenomenon, based on the formation time, different organs can be divided into the central immune tolerance and peripheral tolerance. Peripheral immune tolerance by mature T, B lymphocytes in the peripheral immune organs by clonal deletion clone incompetent, immune neglect and a variety of regulatory T cells formed. Peripheral immune tolerance and human, animal, many diseases are closely related. Physiological immune tolerance after the break, could easily cause a variety of autoimmune diseases, such as systemic lupus erythematosus, multiple sclerosis, type 1 diabetes; avoid graft rejection also need to establish antigen-specific immune tolerance; virus tumor antigen immune tolerance, the prevalence of neoplastic and viral diseases cause the body to the clinical treatment of these diseases is the need to break down the body's immune tolerance to the tumor or viral antigens. Solution of the above problems, we must clarify the the body peripheral immune tolerance maintain or break the cellular and molecular mechanisms of the formation of the premise. Therefore, the establishment of mouse peripheral immune tolerance model-specific non-self antigens, and based on this model, the mechanism of their formation has important theoretical and practical significance. DO11.10 mice by means of molecular biology to BALB / c mice as the background, transferred identification OVA323-339 peptide-specific TCR gene create commonly used immune tolerance in mice. And the mice as a model to do a lot of work in the mucosal immune tolerance, but the strains of mice whole peripheral immune tolerance research reported. To this end, we have carried out this study, the research results obtained are as follows: 1. Passing through the tail vein injection of OVA antigen three times, each time interval 5d use OVA subcutaneous immune. Results show that the test group DO11.10 mice spleen lymphocyte the OVA antigen stimulation proliferative capacity is much lower than the control mice, but its lymphocytes SI value is higher than the same processing as BALB / c mice; FCM The intraoperative measurement results DO11.10 mice of the test group was significantly higher than that of control mice, splenic CD4 ~ CD25 ~ T proportion and quantity to the same processing as BALB / c mice the CD4 ~ CD25 ~ T proportion and quantity changes than DO11.10 mice. The results showed that the success of this study DO11.10 mice the OVA immune tolerance model, the high sensitivity of this mouse model of OVA antigen the manual import TCR gene fragment closely related. Respectively CD4 ~ T cell epitope peptide (OVA323-339, KISQAVHAAHAEINEAG), and the CTL epitope peptide (OVA257-264, SIINFEKL) to stimulate DO11.10 mice spleen lymphocytes - Cellular, the results show, CD4 ~ T cell epitopes peptide stimulation test group lymphocyte proliferation is much lower than the control, corresponding to the CTL epitope peptide results in no significant difference between the test group and the control group; cell epitope peptide the CD4 ~~ T lymphocytes stimulate secretion of TGF-beta Cover far higher, but the experimental group and the control group of mice spleen lymphocytes secrete IL-10 levels did not differ. The results show that the OVA immune tolerance the DO11.10 mice of peripheral immune tolerance model established by inhibiting / anti-OVA-specific T-cell lymphocyte clones, wherein the TGF-β and not the IL-10 play a important role.

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