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Studies on the Preparation of Effective Fractions of Compound Yibazhen and Their Quality Standards

Author: ZhouHongLiang
Tutor: LiuZhiHui
School: Nanjing University of Traditional Chinese Medicine
Course: Pharmacy
Keywords: Wine dogwood meat Fleece-flower root Beggars-ticks Effective parts Preparation Process Quality standards Hypertensive nephropathy
CLC: R286
Type: Master's thesis
Year: 2011
Downloads: 56
Quote: 1
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Abstract

Benign arteriolar nephrosclerosis caused by hypertensive nephropathy essential hypertension (high blood pressure renal atherosclerosis) and malignant small artery renal sclerosis, and accompanied by the corresponding clinical manifestations of the disease. One of the major complications of hypertension, the incidence was 42%, second only to heart complications, is dangerous. Long-term use of chemical drugs for the treatment of hypertension, renal disease easily lead to adverse reactions and tolerability, and contraindications, with certain limitations. Compound a needle is the experience of Jiangsu Province were Chinese Professor Tang Shuhua side by wine dogwood meat, fleece-flower root, Bidens good treatment of hypertensive nephropathy. The subject of the effective parts of the compound a needle screening, extraction, separation and purification, quality standards, and laid the foundation for research and development for the preparation of compound effective parts. The research topics include: 1. Determination of the effective parts by literature and pharmacodynamic studies to determine the wine dogwood meat, fleece-flower root, Bidens effective part of the treatment of hypertension, renal disease, mainly iridoid glycosides, stilbene glycoside total flavonoids. Compound a needle in each Pieces Determination (1) were established Nux glycosides and Morroniside, stilbene glycosides, flavone. (2) the content of the active ingredients of the other side of the Pieces determination, tentatively wine dogwood meat, fleece-flower root, the Bidens Pieces Loganin Morroniside stilbene glycosides, total flavonoid content were in the range of 0.50% -0.90% 0.90% -1.60% 0.90% -2.00% 2.50% -4.50%. Effective part of the preparation process (1) in order to the horse money glycosides and Morroniside, transfer rate (content) as an indicator, wine dogwood meat iridoid glycosides Extraction, separation and purification, drying process to determine the wine the cornelian cherry meat cycloalkenyl ether terpene glycosides effective part of preparation conditions: wine dogwood meat Pieces plus 10 times the amount of water, boiling three times, each time 80 min, the extract was concentrated to a relative density of 1.20 (60 ℃ determination), cooled to room temperature, while stirring, 95% ethanol was slowly added to the alcohol-containing capacity of 60%, allowed to stand overnight and filtration, the filtrate was recovered ethanol until no alcohol flavor after diluted with water to 0.5g/ml ratio by weight of the resin and the amount of crude drug 1:0.75 on the sample solution was added to D101 macroporous resin column on the rate of 2 BV / h, the sample after water elution 5 BV, and then 15% ethanol solution eluted 9 BV and recovery of ethanol, and concentrated to a relative density of 1.05 -1.15 (60 ° C Determination), dried under vacuum at 60 ° C. (2) transfer rate indicators stilbene glycoside, fleece-flower root stilbene glycoside extraction and purification process to determine the conditions of a of stilbene glycoside effective parts preparation process: fleece-flower root Pieces plus 8 times the amount of water, boiling for 3 times, each time of 75 min, the extracts were combined after 80 ° C under reduced pressure and concentrated to a relative density of 1.10 (60 ° C Determination), plus 1 times the extract volume of ethyl acetate and extracted 4 times. (3) The total flavonoid content indicators, extraction of total flavonoids of Bidens purification process was studied to determine the effective parts of the the Bidens total flavonoid preparation conditions: the Bidens Pieces plus 13 times the 70% alcohol extract 3 times, each time 1 h, combined filtrate recovery of ethanol to the alcohol-free taste may Bidens extract, the extract was diluted to 0.5 g / mL, resins and herbs weight ratio 1:0.5,3 BV / h speed on D101 macroporous resin column, the sample water elution 6 BV, and then 50% ethanol solution eluting 9 BV, recovery of ethanol, dried in vacuum at 80 ℃. (4) Preparation of effective parts were the pilot study, the results showed that the screening process is stable and can be used for industrial production. The effective parts quality standards (1) using thin-layer chromatography were established wine dogwood meat iridoid glycosides, system Polygonum multiflorum stilbene glycoside effective parts of the the Bidens total flavonoids and Bidens Pieces TLC identification method. (2) high-performance liquid chromatography, and determination of the the the wine cornelian cherry meat iridoid glycosides site Loganin, the Morroniside content the the tentative Loganin Morroniside total amount shall not be less than 50.0%; determination the system the Polygonum multiflorum stilbene glycoside effective parts of stilbene glycoside content tentative stilbene glycoside content of not less than 55.O%; using colorimetric determination of total flavonoids effective parts of the total flavonoids, tentative total flavonoids effective part of total flavonoids rutin which shall be not less than 50.0%. (3) the effective parts of moisture, residue on ignition, heavy metals, and stilbene glycoside effective parts of ethyl acetate residue in the research, and to develop appropriate content limits.

Studies on the Preparation of Effective Fractions of Compound Yibazhen and Their Quality Standards

Abstract

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