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Objective To observe the musk oolong pill adjuvant arthritis (AA) in synovial lining layer of pathological and basic fibroblast growth factor (bFGF), transforming growth factor-β1 (TGF-β1) expression levels of explore musk oolong pill treatment of rheumatoid arthritis (rheumatoid arthritis, RA) is related to the regulation of bFGF and TGF-β1 levels. Healthy Wistar rats to 30, were randomly divided into normal group (A), model group (B), the the musk Oolong pill group (C), using Freund's complete adjuvant model, musk Oolong pill the after irrigation ig1 weeks, HE staining, light microscopy pathological changes of the various groups of the synovial lining layer, the layers of the lining layer of synovial cells, the immunohistochemistry assay groups synovial lining layer bFGF, TGF-β1 expression. Results 1 synovial lining layer pathological morphology: A group of rats synovial lining layer composed of 1-2 layers of flat or low cuboidal epithelial-like synovial cells and partially continuous synovial tissues without hypertrophy, congestive; B group The proliferation of the synovial lining layer to 4-8 layer was papillary or fence-like protrusions, cells were oval, disorganized, and the increase in size, the synovial tissue hypertrophy, congestion and edema formation of many villous projections protruding into the joint or invasive cartilage and bone; C group lining layers less than that in group B, mild hyperplasia of synovial cells, synovial tissue congestion and edema. 2 synovial lining layer cells storeys and bFGF, TGF-β1 in the determination of results 2.1 synovial lining layers of the number of group B, group C and group A, the number of layers lower in group A, the difference was statistically significance (P lt; 0.01); C group and B group, the number of layers than in group B, the difference was statistically significant (P lt; 0.05). The experimental results show that the musk oolong pill can reduce the cell layers of the synovial lining layer, reduce synovial cell proliferation. 2.2 bFGF expression level of group B, C in group A, bFGF expression than in group A, the difference was statistically significant (P lt; 0.01); C group and the comparison group B than in group B, bFGF Expression, statistical difference significance (P lt; 0.05). The experimental results show that the musk oolong pills can reduce the expression levels of the synovial lining layer of bFGF. 2.3 TGF-β1 expression levels of Group B, C and Group A group, TGF-β1 expression than in group A, the difference was statistically significant (P lt; 0.01); C group and B group, TGF-β1 expression below Group B, the difference was statistically significant (P lt; 0.05). Conclusion The experimental results show that the AA rats after after the musk oolong pill ig1 of weeks, musk oolong pills can significantly reduce synovial tissue congestion and edema and synovial cell proliferation, reduce cell layers of the lining layer, while significantly reducing the lining layer bFGF, TGF -β1 expression levels. Mechanism prompted musk oolong pill treatment of RA may reduce synovial lining layer bFGF, TGF-β1 expression level, inhibition of cell proliferation of the synovial lining layer, reduce pannus formation, and thus to protect the cartilage and bone.
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