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Studies on the Components and the Anti-tumor Activity of Extract from Periploca Forrestii Schltr

Author: LiangYong
Tutor: ZhangHong
School: Sichuan Normal University
Course: Biochemistry and Molecular Biology
Keywords: Black bone vine Chemical composition Antitumor
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 22
Quote: 0
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Abstract


The black bone vine (Periploca forrestii Schltr) is the the Asclepiadaceae (Asclepiadanccae) Periploca genus (Periploca Linn.) Dried stems of plants black keel or the whole plant , located in China 's southwest region , has Qufengchushi , blood Shujin effect. In this thesis, a systematic study of the chemical composition of black bone vine and its preliminary study of the anti- tumor activity . Ethanol extracts after the black bone vines rhizomes sequentially extracted into petroleum ether, chloroform , ethyl acetate , n-butanol , and the aqueous layer five parts , the petroleum ether layer portion by silica gel column chromatography to obtain a crude fragments , and coarse fragments the temperament analysis; chloroform layer portion by silica gel column chromatography , Sephadex LH -20 gel chromatography , combined with a semi - preparative chromatography repeatedly separated and purified , combined recrystallization , extraction and separation of the monomer compound . Nuclear magnetic resonance ( NMR ) and mass spectrometry (MS) and other modern analytical techniques Determination of the chemical structure of the compound after the spectral analysis of the physical and chemical analysis and literature retrieval , structural identification . By cytology experimental method and its preliminary study of anti-tumor activity . Temperament analysis obtained from the petroleum ether layer portion of the black-bone vine analysis 96 chemical components ; chloroform layer portion 7 of a single compound, identified one of the three compounds as ursolic acid , β- sitosterol , hexadecanoic acid . Compounds Ⅰ ~ compound Ⅵ basic anti-tumor activity , compound Ⅶ strong anti-tumor activity , but its too cytotoxic anticancer drugs should not be used directly as a lung cancer A549 cells inhibition experiments .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
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