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β-lactam antibiotics is the molecular structure containing β-lactam ring of the antibiotic, because of its good antibacterial effect, the current animal disease treatment and prevention of the most widely used antibiotics. However, non-standard medication can cause livestock products, especially animal drug residues in milk, detrimental to human health. Therefore, the establishment of a class of efficient, accurate and easy β-lactam antibiotics detection method is particularly important. This paper studies the β-lactam antibiotics in several common drugs such as penicillin G, ampicillin, ceftiofur sodium, cephradine new method of analysis and detection, the main contents are as follows: The first part: the presence of penicillin G hydrogen peroxide oxidation fading reaction of Amaranth, respectively, in the concentration range of two to establish a catalytic kinetic spectrophotometric determination of trace amounts of penicillin G method. Specifically for the low concentration range penicillin G kinetic spectrophotometry, linear range is 0.20 ~ 4.00μg/mL, the regression equation y =-0.015x 0.311 (x: μg / mL), r = 0.9998; higher concentration range Penicillin G inhibitory kinetic spectrophotometry, linear range is 4.00 ~ 50.0μg/mL, the regression equation y = 0.001x 0.280 (x: μg / mL), r = 0.9997. The reaction mechanism was discussed. Methods of measurement wavelength are 520nm, the detection limit is 1.0 × 10-7g/mL, maximum relative standard deviation was 1.23% and the recovery was 96.7% ~ 104.1%. Part II: Creating a surfactant Kinetic spectrophotometric determination of drug residues in milk ampicillin methods. Use neutral medium cationic surfactant Triton100 ampicillin xylenol orange fading reaction catalyzed sensitizing role in pH = 7.0 in Tris-HCL buffer solution, Determination of milk samples ampicillin drug residues, the linear range 0.10 ~ 50.00μg/mL, the regression equation was y = 0.240x 2.151, r = 0.9972, the detection limit is 2.0 × 10-8g/mL, maximum relative standard deviation was 1.15% and the recovery was 98.1% ~ 103.6%. Law for real samples with satisfactory results. This method is simple, fast, accurate, and can be used for a large number of milk samples in the rapid screening and detection of ampicillin. Part III: pH = 7.00 The Britton-Robinson (BR) buffer solution and surfactants polyoxyethylene fatty alcohol ether sulfate (AES) medium, acridine orange - Neutral Red efficient energy transfer can take place, the neutral red fluorescence enhancement. Ceftiofur sodium also makes adding neutral red fluorescence quenching, which established for the determination of ceftiofur sodium new methods. This method for milk ceftiofur sodium determination of drug residues, linear range is 0.10 ~ 60.00μg/mL, the regression equation was y = 399.7x 38.88, r = 0.9980, the detection limit is 0.1 × 10-7g / mL, the maximum relative standard deviation was 1.19% and the recovery was 95.9% ~ 102.7%. Law for real samples and comparison with high performance liquid chromatography with satisfactory results. Experiments show that the method is simple, rapid, sensitive and accurate. Part IV: According to the cephalosporin class of drugs to bovine serum albumin quenching of fluorescence characteristics, the establishment of a determination of cephalosporins in milk two kinds of new methods. Maximum excitation wavelength of the detection system is 280m, the maximum emission wavelength of 341nm. Ceftiofur sodium content (x) is 0.10 ~ 30.00μg/mL time, ΔF with ceftiofur sodium content of linear regression equation was y = 563.1x 360.5, r = 0.9998, ceftiofur sodium detection limit to 0.5 × 10-7g/mL. Cefradine content (x) is 2.50 ~ 80.0μg/mL time, ΔF with cephradine content showed a linear regression equation y = 312x 90.1 (x: μg / mL), r = 0.9999. The determination of cephradine detection limit 1.0 × 10-7g/mL. Experiments show that the method is simple, rapid, sensitive and accurate.
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