Dissertation > Excellent graduate degree dissertation topics show

Northeast Guanzhong anti- swine influenza virus subtype H1N1 effective component

Author: ZouYueHong
Tutor: ZhangYanLong
School: Heilongjiang University
Course: Microbiology
Keywords: Dongbeiguanzhong subtype H1N1 swine influenza virus effective components Madin-Darby canine kidney cell
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 50
Quote: 0
Read: Download Dissertation

Abstract


Dongbeiguanzhong, a perennial evergreen herb recorded in the "Shen Nong’s Herbal", is dry roots and petioles of Dryopteris （Dryopteris crassirhizoma Nakai）. It distributes widely in China especially in Heilongjiang province. Dongbeiguanzhong has become the focus of research at home and abroad, because of its low toxicity and important role in improving uterine smooth muscle and in anti-tumor.In recent years, detailed studies of the morphology, reproductive methods, identification, and pharmacological activity about Dongbeiguanzhong have been reported. But there are few studies of its anti-viral activity and no report on its activity components of the anti-H1N1 subtype swine influenza virus. In order to determine its anti-H1N1 subtype swine influenza activity, 30% and 60% ethanol extracts from Dongbeiguangzhong at safe concentration screened in MDCK cells by MTT method were studied. At the safe concentration, the protection rates of different components for MDCK cell pathologically changed by H1N1 subtype swine influenza virus were determined by cytopathic effect inhibition method, which reflects its anti viral activity in vitro. The results showed that the anti H1N1 subtype swine influenza activity of 60% ethanol fraction which shown related to the concentration was significant while that of the 30% ethanol fraction non significant. Therefore, 60% ethanol component was determined as the main active site of anti H1N1 subtype swine influenza virus.The 60% extract components of Dongbeiguanzhong were isolated by the silica gel column, ODS column, HPLC and so on. Structures of five compounds had been determined by 1H-NMR, 13C-NMR spectroscopy and other methods. They are epicatechin,（Z）-3,5,5-trimethyl-4-（3（3,4,5-trihydroxy-6-（hydroxymethyl）-tetrahydro-2H-pyran-2-yloxy） butylidene ） cyclohex-2-enone,β-rhamno, p-Cumaric acid and catechin.Anti H1N1 subtype swine influenza activity in vitro of picatechin with maximum content in the five compounds was conducted by cytopathic effect inhibition method and MTT colorimetric method. The results showed that epicatechin had significant anti H1N1 subtype swine influenza activity while its protection rate for MDCK cells pathologically induced by H1N1 subtype swine influenza virus as high as 52%. By anti-H1N1 subtype swine influenza active ingredients’screening, active chemical components’isolating and identification, and determination of the activity of mono chemical epicatechin, the anti-viral activity of traditional Chinese medicine Dongbeiguanzhong was further revealed, which provided some important scientific evidence for the research and development of new anti-viral drugs.

Related Dissertations

  1. The Selecting and the Studying of Effective Components Absorbed into Blood from Huang Lian Jie Du Tang,S853.9
  2. Studies on the Preparation Compounds Hedera Nepalensis Injection,S853.7
  3. Study on Effective Composition Extracting and Microencapsulation of Traditional Chinese Medicine,TQ461
  4. The Research of the Total Saponins of Rubus Parviflolius L. Neuroprotective Effect on Cerebral Ischemic Rat’s Model,R285
  5. Extract of Effective Components and Bioactivity Research in Dioscorea Opposita Thunb. Cv. Tiegun,S567.239
  6. Study on Extraction and Purification Process of Effective Components from Docynia Delavayi,TQ461
  7. Chinese Herbal Medicine effective material and basic research methodology design and its application,R284
  8. Elementary Screening for Diuresis Efficiency and Pharmacological Study for Mitigating Proteinuria of WLS in Adriamycin Rat,R285
  9. The Study on the Neurotrophy Mechanism of Qingkailing Effective Composition Antagonizing Focal Cerebral Ischemic Injury in Rats,R285
  10. Molecular Pharmacological Research of Chinese Medicinal Herbs with the Fuction of Replenishing Qi to Invigorate the Spleen on Intestinal Crypt Stem Cells,R285.5
  11. Studyies on Liposome of Astragalus Extract,R284
  12. The Moso people national drug studies,R29
  13. Study on Material Base and Quality Specification of Fruit Malva Verticillate,R284
  14. A Pharmaceutical Study on the Inhibition of Prostatic Hyperplasia by Seeds of Brassica Alba,R285
  15. Extraction and Pharmacodynamic Studies on Effective Components of Chinese Herbal Compounds Gallnut,S948
  16. Simotang chewable main active ingredients and pharmaceutical part of the study,R286
  17. Study on the Effective Components of Reducing Blood Lipid in Semen Cassiae,R284
  18. Studies on Changes of Contents of Effective Components and Their Extraction and Separation in Exotesta of Ginkgo Biloba L.,S792.95
  19. Study on Nutritional Physiology and Effective Components of Officinal Insect-Eupolyphage Sinensis Walker,S899.9
  20. Qingkailing effective component neurons in the cerebral ischemic injury protection mechanisms,R285
  21. Recombinant bovine prion protein separation and purification , and kinky raise hopane extraction of active ingredients , Isolation and structure elucidation,R284

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile