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The Research and Design of the Optical Tweezers System for Cell Manipulation

Author: LiuJing
Tutor: SongZhengXun
School: Changchun University of Science and Technology
Course: Electronic Science and Technology
Keywords: optical tweezers design light trap mobile the dynamic control
CLC: Q2-33
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
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Abstract


The birth of the optical tweezers technology accelerates the research and development of the nano-biotechnology. Compared to traditional observation techniques, optical tweezers has advantage of maintaining cell activity and showing the various stages of a continuous state while the cell under operation and observation. Optical tweezers is a new means of non-physical contacting, no damaging to the tiny particles which is captured and manipulated, provides convenience for future work, as well as in combination with other devices. This article discusses the principle of optical tweezers, the framework of constitute, design and construction, and controlling a moving operation of the captured particles. This paper analyzes two methods of moving the position of optical trap. The relative movement method is using two-dimensional micro-displacement platform to change the position of the optical trap. Another absolute move method is using piezoelectric actuator pan rotating mirror to control the position of the optical trap.According to the basic design of the traditional optical tweezers system, this system can capture and move the yeast cells, and use different numerical aperture of the objective lens to capture the yeast cells, use relative movement method to stably capture yeast cell by optical trap. The relative movement method is suitable for a wide range of mobile. Absolute move method is suitable for small range precision mobile, which is based on designed dynamic control of pan rotating mirror-based optical trap system, analysis of the design of a piezoelectric actuator, transmission of serial data communication between PC and processor. Simulate processor control digital-to-analog conversion chip to output waveform.The future development of optical tweezers technology requires more precision manipulation. It is very necessary to study the optical tweezers system and methods of the two mobile optical trap.

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CLC: > Biological Sciences > Cell Biology > Cell Biology Research Methods > The cytology experimental and cytological studies
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