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The time-resolved fluorescence immunoassay has two different systems:DELFIA and DSLFIA. DSLFIA has more advantages than DELFIA, which is able to directly detect, where the synthesis of novel structure of the chelating agent is critical. The paper successfully synthesized two novel compounds of bipyridine on the basis of th-e chelating agent having been synthesized and a variety of other intermediates. The main works are as follows:1. Starting from the functional requirements of the bifunctional chelating agent, a new compound4,4’-dibromo-6,6’-bis(N,N-bis(t-butoxycarbonylmeth-yl)amino methyl)-2,2’-bipyridine has been synthesized, which simplify the purified process of the product but also avoiding the use of more toxic methanol.2. the novel intermediate compound4,4’-di(p-aminophenylethynyl)-6,6’-bis(N,N-bi s(t-butoxycarbonylmethyl)amino methyl)-2,2’-bipyridine has been synthesized which ov-ercome the incompletely hydrolysis of the ester group, the not ideal effect of fluores-cence intensity, and very low yield factors.3. By IR、1H NMR and FRMS, we confirmed the structure of the above comp-ounds, simultaneously studied the reacted temperature, time, quomodo of adding mat-erial and other factors and improve the yield. This thesis provides an important inter-mediate compound reference for the final synthesis of solid-phase time-resolved fluor-escence immunoassay chelate.
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