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Food to safety first . With economic development , people are increasingly concerned about food safety issues . This paper establishes a capillary electrophoresis method for the determination of melamine residues in food and feed , cyromazine and its metabolite residue analysis of the new method . Chapter One : A brief introduction capillary electrophoresis technology , to summarize the status of research and development at home and abroad for melamine and related drugs , and a brief introduction to the main content of the thesis . Chapter Two : Create a capillary electrophoresis - new method of solid-phase extraction and determination of melamine residue in milk samples . The investigated buffer systems , pH value , concentration , voltage , temperature electrophoresis working conditions with trichloroacetic acid, the solution precipitated protein in the milk samples , and extraction of target analytes , enriched by solid phase extraction column purification ; The best electrophoretic working conditions , melamine in 0.8 -100mg / L range was a good linear relationship (r = 0.9998), the detection limit of 0.12mg/kg , limit of quantification was 0.37 mg / kg . Chapter Three : Create a capillary zone electrophoresis separation and detection of cyromazine and its metabolite melamine . The effects of the sample processing method , and the buffer pH , concentration , voltage , temperature and other electrophoretic conditions were optimized . Through testing, containing 20% NH4OH in acetonitrile extractant to be tested drugs from the actual sample extracted , by ultrasound , centrifugation , nitrogen , blowing a constant volume can be directly on the machine detects . Under optimal conditions , cyromazine and melamine in the range of 0.17-50 mg / L showed a good linear relationship ( r = 0.9998 ) , and the limit of quantification of 0.38 mg / kg in the feed samples , eggs, liquid milk samples limit of quantification of 0.42 mg / kg.
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