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Preparation of the Modified Cole Straw and Its Adsorption Behavior for Proteins

Author: ShiYingYuan
Tutor: LiBuHai
School: Central South University for Nationalities
Course: Analytical Chemistry
Keywords: cole straw modification phosphorylated alkalized LYS BSA BHb
CLC: O647.3
Type: Master's thesis
Year: 2013
Downloads: 17
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Abstract


The proteins have a great significance for biological terms. There is an urgent need tounderstand the mysteries of the proteins in order to apply for all aspects of society.However,based on the characteristics of the biological molecules, the number of stable and pure proteinthat nature can provide is rare, which promotes the research of the separation and purificationmethods for higher purity and better quality proteins.The methods of adsorption to separateand purify protein demonstrate much advantages of simple operation, short processing timeand low cost, thus it is widely used currently.In this thesis the agricultural waste-Cole Straw was used as a raw material.Through thesimple acidification,alkalization processing and the chemical reaction on their surfaces tograft with specific functional groups, the different adsorbents were prepared from cole straw,then its adsorption behavior for lysozyme、bovine serum albumin、bovine hemoglobin wasinvestigated. The specific works are as follows:1. With dimethyl formamide and phosphoric acid as crosslinking and modification agents,repectively, a novel biosorbent was prepared from cole straw. The absorbents werecharacterized by using Fourier transform infrared、 TEM and the X-ray photoelectronspectroscopy. Factors affecting adsorption behavior，such as pH，temperature，contact time，initial lysozyme concentration and NaCl ionic strength were extensively investigated. Resultsshowed that the maximum adsorption capacity of the phoaphorylated cole straw was451.71mg·g-1at pH7.4，25℃and for8h，while the original one gave the largest adsorption capacityof332.43mg·g-1. The seperation and purification of lysozyme from egg white were achieved.The lysozyme was purified19.8-fold in a single step with a activity recovery51.3%.2. Factors affecting adsorption capacity before and after the modification of cole strawsuch as pH, contact time, initial BSA concentration temperature and NaCl ionic strength werestudied. Results showed that the maximum adsorption capacity of the phosphorylated colestraw was127.39mg·g-1at pH=5.0（for unmodified）、pH=6.0（modified）,25℃and for20h,compared to the68.98mg·g-1from unmodified materials. The adsorption phenomena werefound monolayer adsorption following Langmuir isotherm.At last, factors affecting adsorption capacity before and after the modification of cole straw，such affecting factors as pH, contact time, initial BHb concentration temperature andNaCl ionic strength were extensively investigated. Results showed that the maximumadsorption capacity of the phosphorylated cole straw was253.77mg·g-1at pH5.0,25℃andfor20h, compared to the213.75mg·g-1from unmodified materials. The Langmuir isothermfitted better in adsorption process, which showed the adsorption of BHb is monolayeradsorption.3. Sodium hydroxide was used to alkalyze the original cole straw. The absorbents werecharacterized by using Fourier transform infrared and the X-ray photoelectron spectroscopy.Factors affecting adsorption effciency，such as pH，temperature，contact time，initialLysozyme concentration and NaCl ionic strength were extensively investigated. Resultsshowed that the maximum adsorption capacity of the cole straw for lysozyme was702.7mg·g-1at pH7.4，25℃and for8h，while the original one gave the largest adsorption capacityof332.43mg·g-1. The seperation and purification of lysozyme from egg white were achieved.The lysozyme was purified23.44-fold in a single step with an activity recovery60.8%.4. Factors affecting adsorption behavior of the alkalized and unmodified adsorbents，suchas pH, contact time, initial BSA concentration temperature and NaCl ionic strength wereextensively investigated. Results showed that the maximum adsorption capacity of thealkalized cole straw was147.39mg·g-1at pH=5.0（for unmodified）、pH=6.0（modified）,25℃and for20h, compared to the68.98mg·g-1from unmodified materials. Modified cole strawimproves the cellulose microspheres adsorbent performance. The adsorption phenomena werefound monolayer adsorption following Langmuir isotherm.At last, factors affecting adsorption capacity，such as pH, contact time, initial BHbconcentration temperature and NaCl ionic strength were extensively investigated. Resultsshowed that the maximum adsorption capacity of the alkalized cole straw was307.9mg·g-1atpH4.0,25℃and for15h, compared to the213.75mg·g-1from unmodified materials. Theadsorption isotherm was found fitting Langmuir model with monolayer adsorption.5.1.1-allyl,3-methylinidazolium chloride ionic liquid （[Amim] Cl） was synthesized inthe appendix. The products were characterized by FT-IR. Then the dissolution conditions ofcellulose isolated from cole straw in [Amim] Cl was optimized. Chemical modification ofcellulose, isolated from delignified cole straw, was performed in [Amim] Cl ionic liquid with maleic anhydride without catalysts, and was made to adsorp lysozyme.

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CLC: > Mathematical sciences and chemical > Chemistry > Physical Chemistry ( theoretical chemistry ),chemical physics > The physical chemistry of surface phenomena > Adsorption
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