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Pistacia chinensis bunge has a wider geographic distribution, and adaptable. Pistacia chinensis bunge seeds rich in oil, is a good of wild woody oil resources.Recent studies show that the the the pistache seed oil can also be prepared biodiesel, which is important to solve the current energy crisis has.The pistache seed as raw material, to study the pistache oil extraction and its preparation biodiesel process conditions.Preparation of biodiesel to the pistache oil as raw material to provide a theoretical basis for the realization of. If you can take full advantage of Pistacia chinensis bunge oil biodiesel the lower glycerol solution, the production of high-value products.The entire biodiesel industry will be rapid development.1,3-propanediol is an important chemical raw materials and organic intermediates. It is often used to make plasticizers, emulsifiers, surfactants, widely used in pharmaceutical, textile and dyeing industry.1,3-propanediol main purpose is the synthesis of new polyester material-polyethylene terephthalate. It is a polyether, polyester, nylon and other polymers, monomers, and therefore of concern. Poly (trimethylene terephthalate), referred to as the PPT, researchers developed a very promising new polyester material. Because of its excellent flexibility, resilience, fluffy, stain resistance and easy coloring characteristics, it is thus becoming one of the most popular polymer materials.1,3-propanediol, the main production method is the method of ethylene oxide, ethylene aldehyde law and the microbial transformation. Conversion of production of1,3-propanediol due to the chemical synthesis requires high pressure and harsh conditions do not meet the concept of green chemistry, microbiological method has obvious advantages, become the main research directions.The crude glycerol used in this paper is the use of Pistacia chinensis seed manufacturing. Glycerin can not through the purification processing directly used to transformation production1,3-propanediol. In the experiments that use strains for Klebsiella pneumoniae subsp. Pneumonia, purchased from China General Microbiological Culture Collection Center, Numbers:1.1736, In order to increase the production1,3-propanediol ability, The ultraviolet(UV)light and LiCl methods were used to carry out mutagenesis to the Numbers:1.1736. Using this approach,one mutant strains that endured higher concentrations of1,3-PD and yield less byproduct of ethanol and lactic acid were achieved. Original strain shake flask cultures and shake flask fermentation.Under completely anaerobic conditions, to obtain the highest1,3-propanediol yield,14.18g/L, the conversion of glycerol was35.45%.Compared with the original strain, kps271,3-propanediol yield22.16g/L, the conversion of glycerol was55.4%. In fed-batch fermentation mode, reaching62.1g/L; acetic acid mass concentration of58.1%, to reach12.3g/L; the mass concentration of ethanol decreased by39.1%, lower to9.4g/L.
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