Dissertation > Excellent graduate degree dissertation topics show

Study on a Technology Realized the Large-Scale Production about the Comprehensive Development and Utilization of Bovine Blood

Author: JiaJieFeng
Tutor: ChenGuiLin；WangChunJie
School: Inner Mongolia University
Course: Biological Engineering
Keywords: Thrombin Superoxide dismutase Protein hydrolysate bovine blood Comprehensive utilization
CLC: S823
Type: Master's thesis
Year: 2010
Downloads: 56
Quote: 0
Read: Download Dissertation

Abstract


The comprehensive utilization of bovine blood was researched by using the modern technology of industrial biochemistry.The two kinds of highly valuable biochemical product including thrombin and Cu/Zn-superoxide dismutase were isolated from bovine blood.Thus, the bovine blood was reutilized and the environmental pollution due to abandoned blood was solved.A new simple and convenient technology that can be used to realize the large-scale production of copper/zinc superoxide dismutase and thrombin from bovine blood was built in this study. By establishing a fast and simple technology with the tube separator, continuous separation of red cell from blood, the results showed the separation rate reached 1600kg bovine blood/h.About the extraction process of SOD, The process parameters was determined through the experimental study heat denaturing methods under the existing copper zinc salt; On the basis of previous work, The procedure of SOD was built, that includes many technologies, such as continuous separation technology of red cell, heat denaturation, ultrafiltration, acetone deposition and ionic exchange etc.The new procedure abolished any chloroform and alcohol in traditional method of the hemoglobin removed,and When ultrafiltration technology was used to concentrate extracted solution, the acetone use level decreased a lot. The results show, that the most appropriate temperature of thermal denaturation reaction is between 72℃-75℃,and that under the most appropriate temperature, As the copper zinc salt solution concentration increases the effect of thermal denaturation was obviously improved. Finally, the most appropriate conditions of the thermal denaturation process was 72℃and adding dissolve blood volume 3% of copper zinc salt solution.About the procedure of thrombin, this paper develops the method of extraction and purification of thrombin from the bovine plasma by using Ion Exchange. The process is as followings:the plasma is frozen at-20℃and purified by using Ion Exchange. The results show that the recovery of thrombin which plasma is treated with freezing is 48.9%(1.4 times that of the thrombin by using the traditional Ion Exchange), and the specific activity is 1807.9U/mg (1.2times that of the thrombin by using the traditional Ion Exchange).

Related Dissertations

  1. A New Method for Thrombin Detection by Using Aptamers and Gold Nanoparticles,Q55
  2. Study on Extraction and Properties of Superoxide Dismutase from Goose Blood,TS254.9
  3. Shamisen Medicinal and Edible Herbs on glucose and lipid metabolism in the metabolic syndrome and antioxidant effects,R285.5
  4. Berberine on serum levels of superoxide dismutase and malondialdehyde levels in,R743.33
  5. The Expression of TAFI in the Patient with Polycystic Ovary Syndrome,R711.75
  6. The Experimental Study about Protective Effects of Puerarin on Liver of Brain-dead Rats,R285
  7. Experimental study of the skin soup chloasma mouse model of tyrosine , SOD and morphological effects,R285
  8. Study of the sugar grams decoction oxidative stress and pancreatic β cell function in type 2 diabetic rats,R285
  9. Aptamer Conformational Change and Quantum Dots Imaging in Single-Molecule Level,Q52
  10. Study on Polysialylatin of CuZn-Superoxide Dismutase,TQ460.1
  11. Synthesis, Characterization and Biolgical Activities of Superoxide Dismutase and Peroxidase Model Complexes,O641.4
  12. Low-frequency Repetitive Transcranial Magnetic Stimulation on ATP Enzymes, MDA and SOD of Central Nervous System in Immune-mediated Motor Neuron Disease Guinea Pig,R744
  13. Continue Irrigation after Arthroscopy Impact the Knee OA on SOD, NO Levels in Animal Experiment and Clinical Research,R684.3
  14. The Effect of Magnetic Field on Serum Free Radicle in Patient Undergoing Operation for Pituitary Tumor,R614
  15. Alcohol consumption on cardiac structure, function, and serum levels of oxidative stress factor,R541
  16. The Study of the Effect of Rhubarb Given at Different Time during the Perioperative Period on Intestinal Mucosal Barrier Function in Rat after Partial Hepatectomy,R285.5
  17. Shugan Qi Tang on isolated systolic hypertension ( qi deficiency ) clinical efficacy and serum CAT, GSH-PX, SOD, MDA impact,R259
  18. N-acetylcysteine ??for BDE-209 in neonatal rat hippocampal neurons oxidative damage and preliminary study BDE-209 on hippocampal neurons influence the level of DNA methylation,R114
  19. Chuxiong Economic Development Zone Planning for a new industrial park based on circular economy,F205
  20. Effects of Phenolic Compounds on Vitellogenin Induction and Enzymes Activities in Goldfish,X174
  21. Effect of Hg2+, Pb2+, Cd2+ on the T-SOD Activity and Mitochondrial COI Gene of Calanus Sinicus,X174

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Cow
© 2012 www.DissertationTopic.Net  Mobile