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Rice Drf2, A Transcriptional Activator, Is Involved in Leaf Wax Synthesis

Author: WangYouHua
Tutor: HuangRongFeng
School: Chinese Academy of Agricultural Sciences
Course: Biochemistry and Molecular Biology
Keywords: Waxy Transcription factor Rice Drought Stress
CLC: Q943
Type: Master's thesis
Year: 2010
Downloads: 148
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Abstract

Plant wax in the synthesis of epidermal cells secreted into the interior of the plant cuticle and the surface to form a barrier between the plants with the outside world, in order to protect the plant from outside biological and non-biological attack. A large number of genes involved in the formation process of the waxy waxy regulation of synthesis and transport in the process. Transcription factor plays an important role in the plant response to external stress, can regulate the expression of multiple genes related. Studies have shown that, through the manipulation of a transcription factor that cities function genes play a role in allowing plants to resist external stress good traits. Ethylene response transcription factor (ERF) stress plays an important role in signal transduction in plants adversity. Research reports indicate that a variety of plant ERF family of transcription factors to regulate the synthesis of the plant cuticle wax to improve the performance of plant drought. Rice massively parallel sequencing (MPSS), the study results from the 12 drought-stress-induced drought response factor (Drought Response Factor, DRF) and selected by salt and ABA-induced increase ERF transcription factor DRF2 of. Bioinformatics analysis showed, DRF2 to contain a AP2/ERF conserved domains, the analysis showed that the transcription factor belonging to the drought response element binding protein superfamily of ERF subfamily members are AP2/ERF transcription factor, the transcription factor AP2 domain sequence characteristics The DREB classes. NCBI Blast software results showed that the waxy synthesis of DRF2 Arabidopsis transcription factor gene SHN1/WIN1 sequence similarity up to 65%. DRF2 start codon upstream 2000bp sequence with GUS fusion analysis of the characteristics of its expression in Arabidopsis. The results showed that the DRF2 Arabidopsis stems, leaves, pods, flowers have a strong expression, and almost no expression in the root, this result with the rice DRF2 tissue-specific expression is consistent. Using yeast transient expression of qualitative analysis and rice callus subcellular localization prove the, DRF2 localized in the nucleus, and has the characteristics of self-activated. Showed that the gene is a typical ERF transcription factor. For the study of the function of the gene, we created overexpression DRF2 Arabidopsis and rice, the interference DRF2 rice plants. The experimental results show that overexpression DRF2 Arabidopsis leaf surface wax content increases, improved drought tolerance, reduce the rate of water loss. The overexpression DRF2 enhanced Arabidopsis wax synthesis and stress-related gene expression. Similar to Arabidopsis, overexpression synthetic wax DRF2 Rice and stress-related genes were up-regulated, RNA interference DRF2 rice these genes showed down. The scanning electron microscope showed that DRF2 regulation of leaf surface waxy synthesis; interfere with the plant's leaves decreased water retention capacity; further by gas chromatography - mass spectrometry (GC-MS) analysis of wax components, results show that the DRF2 may in regulation waxy long-chain fatty acids, aliphatic hydrocarbons and waxy substance to exercise an important function. At the same time, the results found that over-expression of The rice Stress related gene expression in DRF2 interference DRF2 lower part of the stress-related gene expression. Therefore, the study showed that rice DRF2 is regulation of leaf surface wax biosynthesis.

Rice Drf2, A Transcriptional Activator, Is Involved in Leaf Wax Synthesis

Abstract

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