Dissertation > Excellent graduate degree dissertation topics show

Study of Braided Silk Surgical Suture with Antibacterial Property

Author: LvAiFeng
Tutor: WangZuo
School: Donghua University
Course: Textile Engineering
Keywords: SurgicalSite Infections (SSIs) Suture Silk Braided Antibacterial Sustained-release
CLC: TS106.42
Type: Master's thesis
Year: 2013
Downloads: 31
Quote: 0
Read: Download Dissertation

Abstract


Braided silk surgical suture has become one of excellent and widely used sutures in the world for good performances of silk and its stable, easy-handle braided structure. But bacteria prefer to adhere to the silk material and get into the crack of braided sutures, increasing the risk of Surgical Site Infections (SSIs). SSIS not only make the patient much more painful for the delay of wound healing, and increase their, even the society’s, economy burdens. If suture is antimicrobial and can prevent bacteria attaching and growing onto, it could reduce the probability of SSIs.For these reasons, the study of braided silk surgical suture with antimicrobial property has a great important value on clinical application.In the paper, ZL was chosen as the antimicrobial agent. Firstly, braided silk suture were dipped in antimicrobial agent ZL. Then sutures before and after treatment were tested for in vitro efficacy against Staphylococcus aureus and Escherichia coli by a zone of inhibition assay to determine whether the agent ZL was useful.Secondly, sutures were evaluated for sustained antimicrobial efficacy, compared with commercial antimicrobial sutures, and for stability after24-h aqueous immersion and ethylene oxide sterilization. Thirdly, sutures were coated with three kind of antimicrobial treatment agents ZLS, ZLC and ZLP respectively which were made from sustained-release materials fibroin, chitosan and Polycaprolactone. Finally, the best sustained-release material and the better antibacterial coating process was screen out by analyzing the effects of different parameters on coating, SEM analysis, agent release assay, and evaluating the physical and mechanical properties.The results of the study show that:Sutures with ZL exhibited distinct zones of inhibition against Staphylococcus aureus and Escherichia coli. But the antibacterial duration was shorter, the longest for threedays, while Coated Vicryl(?)Plus Antibacterial Suture for7days.The antimicrobial performance of Suture with ZL was unstable to water for the zone of suture after water immersion greatly reduced.The zones of Suture with ZL after ethylene oxide sterilization against staphylococcus aureus, Escherichia coli was reduced by18.36% and3.39%apartly, but antibacterial continuous days were changeless, which showed that ethylene oxide sterilization had less effect on the antimicrobial performance of suture with ZL.The antimicrobial property of sutures with ZLS and ZLC didn’t make an improvement, while suture with ZLP demonstrated good sustained antimicrobial efficacy for its zone maintaining for7days as same as commercial antibacterial.The surface of suture with ZLP was smooth comparing with untreated suture and no bacteria adhere on after being inoculated on Staphylococcus aureus by observing in the SEM.The results of agent release assay were same as antibacterial durability test. ZLP can control ZL releasing slowly from suture and can make suture maintain a long antimicrobial effect. The concentration of ZL released from suture was less than the blood medicine peak concentration, in a safe range.After coating sutures with rollers, the coating was thin and uniform with antibacterial duration decreasing. But it can be improved through increasing the times of coating, the molecular weight of PCL and PCL concentration. Comprehensive consideration of the antibacterial durability, quality and diameter changes after coating, the optimal process was determined and was18/7.5(5), namely PCL molecular weight was180000, PCL concentration was7.5%and the coating times were5.Suture coated with ZLP was even and had no hairiness on the surface. The gaps of braided structure reduced and got closer. After coating, suture diameter increased a little and the tensile strength of suture declined, but still met requirement; The Initial modulus increased but was still less than commercial suture’s, maintained the silk material pliable advantage and has no effect on the operation using.In conclusion, antibacterial ZL demonstrated the excellent antibacterial properties when applied on sutures. After adding slow-release material PCL, its antibacterial duration was longer and produced similar sustained antimicrobial efficacy against S.aureus as Coated Vicryl (?) Plus Antibacterial Suture. Antibacterial agent released slowly with low concentration and was non-toxic. After coating, suture had smoother surface and the changes of its gauge, tensile strength, and flexibility were acceptable and conformed the suture using standard.

Related Dissertations

  1. Fabrication of Membrane Electrode Assembly for PEMFC,TM911.4
  2. Study on the Syntheses and Antibacterial Acticity of Metal Organic Complexes Built from Three Carboxylate Ligands,O621.13
  3. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  4. Properties and Application Research of Multi-Antibacterial Polyamide Fibers,TQ342.1
  5. Preparation and Properties of Chitosan-based Thermosensitive Composite Hydrogel,R943
  6. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  7. Study of Isolation and Identification on Antimicrobial Substances Produced by Lactobacillus Acidophilus NX2-6,TS201.3
  8. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  9. Study on the Enrichment of Antimicrobial Peptides and Purification Technology in Wheat Germ,TQ936.16
  10. Study on Screening of Bacterial Quorum Sensing from Plant Extraction and Their Inhibitions on Biofilm Formation,Q93
  11. Development of Functional Automotive Interior Textiles,TS106.6
  12. Initial Studies on the Antibacterical Activity in Vitro and the Safety Evaluation of Propolis Extraction,R285
  13. The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell,R739.8
  14. Application Adipose-devired Stem Cells and Silk Fibroin and Chitosan Scaffold Materials Building Common Cells-stents Complex Experiments,R329
  15. East Second District Mountain gas field 1 section and box 8 Sedimentary facies and reservoir evaluation,P618.13
  16. Oxya endogenous inhibitory protein isolates SDLH feasibility study as a preservative,TS202.3
  17. Based on green fluorescent gene targeting studies of transgenic silkworm silk,Q78
  18. More than enough calcium and magnesium metal ligand complexes Synthesis, Structural Characterization and Antibacterial Activity,O641.4
  19. Schiff base Synthesis, Characterization and Antibacterial Properties,O641.4
  20. Research on the Mechanism of Polyester Silk-like Fabric Based on Differential Polyester Fiber,TS151
  21. Development of Electronic Real-Time Inspecting System for Eaw Silk Based on NI cRIO,TS147

CLC: > Industrial Technology > Light industry,handicrafts > Textile industry,dyeing and finishing industry > General issues > Blended fabric > Yarn > Line
© 2012 www.DissertationTopic.Net  Mobile