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Studies on Screening for Resistance Source, Gene Mapping and the Expression of Resistant Related Genes to P. Sojae in Soybean

Author: HuGuanJun
Tutor: XingHan
School: Nanjing Agricultural College
Course: Crop Genetics and Breeding
Keywords: Soybean [Glycine max (L.) Merr.] Phytophthora root rot Resistanceidentification Genetic mapping Resistance-related genes
CLC: S435.651
Type: Master's thesis
Year: 2013
Downloads: 5
Quote: 0
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Abstract


Phytophthora root rot (PRR) caused by Phytophthora sojae (P. sojae) is a soil-borne disease, and has a serious impact to the world’s soybean production, especially in the United States, Brazil, Argentina, China. The races of Phytophthora sojae evolves so fast, that different dominant races exit in different regions. The most effective method to prevent the disease is breeding resistant varieties, of which the main method is breeding resistant cultivars of complete resistant. Screening resistance sources and finding new resistant genes are the foundation and key to breeding resistant cultivars. Soybean-P. sojae interaction accord with gene to gene relationship. The resistance genes of soybean can be preliminary postulated based on the gene to the gene theory. Hybrid combinations and genetic analysis could help to dig out new resistant gene. All of this is effective for shorten breeding periods.The main goals of this study were:(1) to find out the new complete resistant cultivars to P. sojae and the cultivars’ distribution (2) to investigate genetic mechanism of complete resistance to P. sojae and linkage analysis of SSRs (3) to understand the expression mechanism of resistance-related genes. The main results are as follows:1. Screening completely resistance sources.196soybean cultivars or lines were identified for their responses to6different races of P. sojae, throught the hypocotyls inoculation. All of these cultivars or lines produce29different reaction types with the6races of the P. sojae. Among them, there are3reaction types accorded with that of single gene, and the others were new reaction types. There were7cultivars probably had gene Rps6,3cultivars probably had gene Rps2,4cultivar probably had gene Rps4or Rps5or Rps7. Among the196cultivars, the resistance cultivars in Central China were the most abundant, especially in Hubei and Si Chuan province.2. Inheritance analysis and linkage Analysis of SSRs of complete resistance. Chinese soybean cultivar (line) Dafangliuyuezao had the different reaction to the10P. sojae isolates and could carried new resistant gene. The F2:3population derived from Dafangliuyuezao (resistant)×Aijiaozao (susceptible) were identified the gene for resistance to P. sojae and mapped the gene to linkage groups. The results showed that the complete resistances in Dafangliuyuezao was controlled by a single dominant gene (Rps), and the resistance gene was temporarily designated RpsAH which was located in the molecular linkage group N and closely linked with Sat-166.3. Expression analysis of resistance-related genes. Reactive Oxygen Species, PR, transcription factors, signal transduction genes are very important for resistant response of plant. We investigate the expression of these genes in two cultivars which have different response to P. sojae. to explore their contribution to soybean-P. sojae interaction.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Oil Crop pests and diseases > Soybean pests and diseases
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