Dissertation > Excellent graduate degree dissertation topics show

Preparative Separation of the Herbicidal Substance from Pestalotiopsis Microspora PM-1Isolate

Author: Wang
Tutor: DongJinGao
School: Agricultural University of Hebei
Course: Microbial and Biochemical Pharmacy
Keywords: Pestalotiopsis microspora High-speed counter-current chromatography Separation Herbicidal activity
CLC: S482.49
Type: Master's thesis
Year: 2013
Downloads: 21
Quote: 0
Read: Download Dissertation


One strain was isolated from the infected leaves of bamboo and it was determined tobe Pestalotiopsis microspora PM-1by the molecular and morphological identification. Theprevious research showed that the crude extracts from Pestalotiopsis microspora PM-1hadhigher herbicidal activity on weeds and might be developed to be bioherbicide. Thisresearch is focus on separating and purification of the herbicidal fractions and the mainresults are as f ollows:1. PD media (Potato dextrose broth) was used to culture the Pestalotiopsis microsporaPM-1, and the filtrate liquid was extracted by ethyl acetate with the same volume andevaporated under reduced pressure to obtain the crude extracts. The high-speedcountercurrent chromatography (high speed counter current chromatography, HSCCC) wereused for separation and purification of the crude extracts. In order to select the best HSCCCsolvent system for crude extracts,10kinds of solvent systems were prepared according tothe method designed by Oka. The results showed that the method of seed germination wassuitable to determine the partition coefficient of the solvent system. The results alsoshowed that No.7solvent system was more suitable for HSCCC separation of crude extracts.The ethyl acetate crude extracts of PM-1were separated by HSCCC with the two-phasesolvent system consisting of n-hexane-ethyl acetate-methanol-water-solution (4:5:4:5) andthe results showed that the crude extracts can be preliminarily separated as Ⅰ、Ⅱ、Ⅲ andⅣ four componentsof whichfractionⅡ had the most intensive herbicidal activity onDigitaria sanguinalis and was further analyzed by high performance liquid chromatography(HPLC) on C18column with acetonitrile:water=75:25(V/V) as the mobile phase underisocratic elution. Fraction A with the retention time of7.954min showed an effectiveherbicidal activity on seed germination of Digitaria sanguinalis and theby DAD analysisindicated that fraction A is a pure compound.2. The crude extracts were separated by silica gel column chromatograph, and HPLC. The results showed that the crude extracts can be separated for two components. Fraction Bwith the retention time of3.510min has the most herbicidal activities, and it was proved tobe a pure compound by. the DAD analysis.)

Related Dissertations

  1. The Flow Theroy Investigation of Compressor Cascade with Split,TK474.8
  2. Synthesis and Resolution Property of N-(9-Fluorenylmethoxycarbonyl)-D-Tryptophan and D-Phenylalanine Imprinted Polymer,O631.3
  3. Experimental Study and Analyses of O2 -CO2 Production Based on Chemical Looping Method,O611.3
  4. Screening of Plant Pathogen Inhibition of Plant Extracts,S482.2
  5. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  6. Study on Separation、 Preconcentration and Analysis of Trace Elements with Carbon Nanometer Materials,TB383.1
  7. The Research on the Microwave Stability of Selenium-Enriched Rich Bram,TS210.9
  8. Preparation of Polysulfonate Ultrafiltration Membrane and Application on Oil Decolorization,TQ320.721
  9. Studies on the Extraction, Saparation and Purification of Red Pigment and Procyanidin of Red Skin of Peanuts,TS202.3
  10. Study on the Characteristics and Simulation of the Hydrocyclone Reparation Process for Initial Rainwater,X522
  11. To Study the Techniques for Purification of Nature Products by High-speed Counter-current Chromatography(Ⅱ),R284
  12. Phosphate modification of nano- TiO 2 Photoelectrochemical Properties of the,TB383.1
  13. Isolation of Highly Purified Cellulose from Wheat Straw and Its Application,X712
  14. Chiral Resolution of Naproxen by Lipase in Water-miscible Organic-solvents,TQ463
  15. Studies on Purification and Properties of Antifungal Compound Produced by Streptomyces Sp.182-2,S482.2
  16. Gansu L TV Station to Implement Separation of Production and Broadcasting System Obstacle and Breakthrough of Path Analysis,G229.2-F
  17. Metallic and semiconducting carbon nanotube density gradient separation,TB383.1
  18. Preparation and Physicochemical Properties of ACE-inhibitory Peptides from Silkworm Pupae Protein,S886.9
  19. Purification and Identification of an Acidic Polysaccharide Fraction GFLA-1 from the Flowers of Panax Ginseng C. A. Meyer,S567.51
  20. RAFT Soap-free Emulsion Polymerization and Preparation of Binds with ABA Triblock Structures,TS190.2
  21. Method Study of Activity Non-destructive Inspection Based on Phase Contrast Microscopic Image of Neuron,TP391.41

CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Herbicides ( herbicide ) > Other herbicides
© 2012 www.DissertationTopic.Net  Mobile