Dissertation > Excellent graduate degree dissertation topics show

Study on Separation Performance of L-Arabinose in Corn Husk Hydrolyzate by Zymotechnics

Author: ZouHongFei
Tutor: ZhangZeSheng
School: Tianjin University of Science and Technology
Course: Nutrition and Food Hygiene
Keywords: corn husk hydrolyzate xylose glucose L-arabinose
CLC: R151.2
Type: Master's thesis
Year: 2012
Downloads: 29
Quote: 0
Read: Download Dissertation


L-arabinose is one of the new functional monosaccharide, which plays a role in controling blood-sugar content, inhibiting fat formation and proliferating of Bifidobacterium, etc. Extracting highly purified L-arabinose quickly and efficiently has become a hot research. Hemicellulose acidolysis has been widely used to produce L-arabinose in industry currently. The product is a kind of mixture contained several monosaccharides. The current method of using resin or thin layer chromatography to extract L-arabinose is complicated and inefficient. This text studied on separation of L-arabinose by microorganism zymotechnics. Corn husk hydrolyzate contained three primary monosaccharides, namely glucose, xylose and L-arabinose. This experiment investigated the circumstances of the three monosaccharides utilized by different microorganism.First of all, raw material and the method of preliminary extraction were screened. Sulfuric acid was used to hydrolyze corn husk to prepare hydrolyzate. HPLC was used to determination the content of main monosaccharides in corn husk hydrolyzate. The results showed glucose, xylose and L-arabinose contained6.11%,18.82%and11.34%, respectively. And according to this, a analog culture medium was prepared.Afterwards, common five strains yeast, seven strains mould, seven strains bacteria, four strains lactobacillus and Mgbl, Mgb2which were separated and screened from environment were fermented in analog culture medium. Medium was detected by HPLC during fermentation. The results indicated that glucose could be consumed completely by all bacterial strains within1-2days. Bacteria and lactobacillus were not suite for separation of xylose and L-arabinose. Torulopsis spp., Trichoderma viricle, Botrytis cinerea, Mgbl and Mgb2have good selectivity to separating xylose and L-arabinose. Most xylose can be consumed by these five strains after3days fermentation. Residual rate were only5.8%,2%,5.2%,4.5%and3.9%respectively. While L-arabinose still remained substantially. Retention rate were83.4%,70%,76.8%,88.6%and77.7%respectively.Corn husk hydrolyzate was fermented by Torulopsi spp., Trichoderma viride、Botrytis cinerea, Mgb1and Mgb2which were screened by analog culture medium and detected by IIPLC during fermentation. The results showed that residual rate of xylose was less than5%after3days fermentation by Torulopsi spp.,4days fermentation by other4strains, while the retention rate of L-arabinose was more than75%. This separation method is simple and efficient. Tondopsis spp., Trichoderma viride and Botrytis cinerea are common and accessible. It provided reference for separation of xylose and L-arabinose by microorganism zymotechnics some extent.

Related Dissertations

  1. Expression of Glucose Oxidase Gene from Aspergillus Niger Z-25 in Pichia Pastoris,Q78
  2. The Changes of Intimal Macrophages, Smooth Muscle Cells, Collagen, and Cpt-Ⅰ, GLUT-4 of Vascular Media in Human Lower Limb Artery with Atherosclerotic Stenosis,R543.5
  3. Effect of High Glucose on Chemokine Ligand 16 (CXCL16) Expression and Secretion in THP-1 Macrophages and Intervention of Aspirin in the Activity,R587.2
  4. Effect of β-Casomorphin7 or Casein Peptides on Blood Glucose and Oxidative Stress of the Diabetic Rats,R587.1
  5. Effects of Angiopoietin-1 on Vascular Angiogenesis Regulator Factor in Human Umbilical Vein Endothelial Cells Cultured with High Glucose,R587.2
  6. Efficacy and Safety of Biphasic Insulin Aspart 30 Once-daily Treatment in Patients with Type 2 Diabetes,R587.1
  7. The Blood Glucose Control and Influencing Factors in Type 2 Diabetic Patients with Oral Hypoglycemic Agents,R587.1
  8. Construction of Engineering Strain Saccharomyces Cerevisiae Metabolizing Xylose,Q78
  9. Study on Synthesis of Xyloses Esters and Their Application in Cigarette,TQ655
  10. Effects of Angiopoietin-1 on Intercellular Junctional Protein in Human Umbilical Vein Endothelial Cells Cultured with High Glucose,R363
  11. Construction of Protic Ionic Liquid Modified Electrode and Its Enhanced Action in Direct Electron Transfer of GOD,O646
  12. The Marrlid Reaction of Chitosan Oligsccarids and the Antioxidant Activity of It’s Derivatives,R285
  13. Effects of Salt and Alkali Stresses on Photosynthesis and Saccharometabolism of Chloris Virgata Seedlings,Q945.78
  14. Study on Hydrolysis of Cellulose by Polyoxometalate,TQ352.1
  15. α-MSH Influences the Excitability of Glucosensing Neurons in Rat Hypothalamus,R338.2
  16. Effects of Antipsychotics Treatment on Blood Glucose of Patients with Schizophrenia,R749.3
  17. The Metabolism of Glucose and Lipid in the Patients with Polycystic Ovarian Syndrome,R711.75
  18. Study on the Ethanol Fermentation of Hydrolysis of Biomass,TK6
  19. Effects of Sleeve Gastrectomy on Blood Glucose, Gastrointestinal Hormones and Serum Lipid in Goto-Kakizaki Rats,R656.6
  20. Ethanol Fermentation of Xylose by High Density Limiting Oxygen Yeast and Mechanism of Yeast Stress Ethanol Tolerance,TQ223.122
  21. The Effect and Underlying Mechanism of Resveratrol- mediated Cellular Calcium and Glucose Utilization in Rat Cortical Neurons,R285.5

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition > Nutritional Biochemistry
© 2012 www.DissertationTopic.Net  Mobile