Dissertation > Excellent graduate degree dissertation topics show

Studies on Active Ingredients and Fingerprint of Ganoderma

Author: ZhangShengLong
Tutor: XieXiaoMei
School: Anhui University of Chinese Medicine
Course: Pharmacognosy
Keywords: Ganoderma Active polysaccharides Triterpenoid Nucleosides HPLC Fingerprint Similarity
CLC: R284.1
Type: Master's thesis
Year: 2013
Downloads: 33
Quote: 0
Read: Download Dissertation

Abstract


The present situation of Ganoderma cultivation is multi–species in our country, andcultivation methods and conditions are different, at the same time, geographicalenvironment, climate, temperature and other factors vary greatly. It is not clear if thesefactors will affect the nutrients and active substances of the fruiting body, and bringabout changes to the quality of Ganoderma lucidum. Therefore, it is necessary toestablish the detection method of the active ingredient in order to examine the influencedegree of the active ingredient.It is first time to establish a HPLC method for detecting GLIS, which is an activepolysaccharides with high molecular weight isolated from the fruiting bodies ofGanoderma lucidum. The polysaccharide GLIS was separated with the other ingredientsusing HPLC analysis, methodological study test showed good repeatability, highprecision, good reproducibility, which is suitable for the quantitative analysis of theactive polysaccharide of Ganoderma. Ganoderma samples of different sources wereanalyzed, the results showed that only a portion of Ganoderma lucidum contains theactive polysaccharide GLIS. But it does not detected in the Ganoderma sinense,Ganoderma applanatum, Ganoderma tenus and Ganoderma tsugae.The chromatographic conditions of HPLC fingerprint are optimized by comparingdifferent column and mobile phase. The optimized HPLC fingerprint contained acidicGanoderma triterpenoid peaks and neutral Ganoderma triterpenoid peaks. Themethodological study results show it had good repeatability, precision andreproducibility with the compliance analysis requirements for fingerprint analysis.Different sources of Ganoderma sample were analyzed using the fingerprint method theresults showed the similarity of Ganoderma different species fingerprint is very low,and its main triterpenoid ingredient content was significantly different. Some of the different varieties of samples had low similarity, and there also some samples had highsimilarity. The similarity between different cultivation methods, cultivation locations,cultivation of age or harvest time harvest Ganoderma sample are very high, indicatingthat these factors did not effect the content of Ganoderma Triterpenes.HPLC method was established to detect the content of uracil nucleosides, uridine,adenine, and guanosine of Ganoderma. This method is simple, rapid, and good stabilityand repeatability. The contents of four nucleosides from different species of Ganodermahad significant difference through HPLC analysis, there are also significant differencesin varies species of Ganoderma. HPLC fingerprint analysis method of Ganodermanucleotidoids were established, and was used to detect different sources of Ganoderma,it is found that the content of nucleosides significantly reduced with the extension ofspore powder collecting time, particularly the stain sample of109, which is differentwith the results of triterpenoid. The similarity of Ganoderma species such asGanoderma sinense, Ganoderma applanatum, Ganoderma tsugae, Ganoderma tenusfingerprint is lower, these factor of different cultivars, cultivation time, place ofcultivation had effect on the content of Ganoderma nucleosides.The quantitative determination HPLC method of active polysaccharide were firstestablished, HPLC triterpenes fingerprint condition of were optimization, and HPLCnucleosides fingerprints was first established to detect different sources of Ganoderma.These research establish a basis for the Ganoderma quality control.

Related Dissertations

  1. Syntactic Features Based Pronoun Resolution,TP391.1
  2. Research of IRC Botnet Detection Based on Behavior,TP393.08
  3. Chemical Structures and Mechanism Research of Anti-aging Leading Compounds from Natural Products,R285.5
  4. Study on the Pharmacokinetics of Xiaoaiping and Chlorogenic Acid in Rats and the Quality Standard of Xiaoaiping Preparations,R285
  5. Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
  6. Development of Detection Methods of Effective Biological Components from Tea by High Performance Liquid Chromatography and Their Applications in the Analysis of Fenghuang Oolong Tea,TS272.7
  7. Studies on the Residues of Bromoxynil and Mesotrione in Maize and Soil,S481.8
  8. Production of Monoclonal Antibodies Against Thiabendazole and Development of Heterologos Elisa,S482.2
  9. Comprehensive Evaluation of Flue-cured Tobacco Quality in Pingdingshan and Comparative Analysis with American Tobacco,S572
  10. Molecular Cloning and Differential Expression of Isopentenyl-Diphosphate Delta Isomerase 1 Gene from the Fungus Ganoderma Lucidum,S567.31
  11. Study on Synthetic of BINOL, Amino Acid and Derivatives Molecularly Imprinted Polymers and Their Application in the Chiral Resolution,O631.3
  12. Analysis of Effective Biological Components in Chinese Commerical Tea Drinks,TS272.7
  13. Injection with four commonly cefathiamidine Compatible Stability of transfusion,R942
  14. Application IP-RP-HPLC method study of creatine phosphate in mice pharmacokinetics and metabolic disposition,R96
  15. Mycobacterium smegmatis MSMEG 6 402 gene function,R378
  16. Mezlocillin sodium infusion with four commonly Compatible Stability,R942
  17. Injection piperacillin sodium and tazobactam sodium content determination and compatible stability of visits,R927.2
  18. WordNet and the \,G254
  19. In Some Areas of Qinghai Cordyceps Sinensis RAPD Analysis of Genetic Diversity,S567.35
  20. Lie Symmetry, Simiarity Reduction of (2+1)-Dimensional Generalized Burgers Equation,O175.29
  21. Study on Determination of Urinary Metabolites of Toluene, Styrene, N-Hexane by HPLC,O657.72

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
© 2012 www.DissertationTopic.Net  Mobile