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Shenguiren granules is clinical experience and composed of9herbs, such as ginseng, angelica, and so on. Be good for our body or beneficial blood and brain. But because of many original formula, and the traditional decoction are inconvenient to save and carry. In order to reduce the ingredients for preparation, this paper based on guarantee its effect on prescription composition optimization, at the same time, the study on preparation process and quality standard to establish the fingerprint and improve the quality standard.The original prescription from ginseng, angelica and other9kinds of medicine composition, if take the full orthogonal experimental design to study of the components of great workload, therefore, the obtained T derived from the orthogonal experimental design method to study on prescription of the Shenguiren granules. The9kinds of Chinese herbal medicine is designed into9factors, each factor is provided with and without the two level, divided into12drug combination group by animal experiment, animal model for two. The red blood cell (RBC), hemoglobin (HB) content of blood rheology and blood stasis rats as detection index, Results:ginseng, Chinese angelica, Suanzaoren compatibility of RBC content7.20±0.89(1012·L-1), HB content125.3±9.1(pg·mL-1), blood rheology index statistics show no significant difference with the original prescription, Show similar efficacy, determine the ginseng, angelica, Suanzaoren compatibility prescription for the optimal prescription.Will the new prescription decoction used in clinical, A statistical analysis of74cases showed,36cases were cured, accounting for48.6%, effective in18cases, accounting for24.3%, effective in17cases, accounting for23%, invalid3cases, accounting for4.1%, the total effective rate was95.9%. Curative effect, The prescription has obvious blood, invigorating Qi, promoting blood circulation and removing blood stasis role.In order to determine the formulation and preparation technique, the new prescription in-depth analysis, prification process and related pharmacological indexes were observed by, Results of preliminary experiments show that the granule accords with requirement, and good taste., Therefore the choice of granule is the prescription type. Orthogonal test was used to study on the preparation process cf the ney prescription, preparation process and technology parameters are obtained. And the new preparation named parameters normalized kernel particle.For the research on the quality standard of shenguiren granules, in order to control the quality of preparation, ensure clinical efficacy. According to the ginseng, angelica, semen Ziziphi Spinosae was identified by TLC study,, TLC identification condition of three kinds of medicinal materials ginseng qui particles in the, According to "Chinese Pharmacopoeia"2010Edition (a) appendix a method to determine the moisture content, particle size, solubility, microbial limit etc.. Method for simultaneous determination of Re, Rbl ginsenoside Rgl, HPLC, the content shall not be less than1.440,0.930,1.200mg·g-1. Formulate quality standard for shenguiren granules.In order to improve the quality standard, the HPLC fingerprint of research of shenguiren granules by HPLC, The chromatographic column, the mobile phase, the detection wavelength, column temperature, flow rate of chromatographic conditions, To determine the parameters normalized kernel particle HPLC fingerprint chromatography conditions:Chromatographic column:Dimma Diamosil C18(250mm×4.6mm,5μm); the mobile phase acetonitrile-water system:gradient elution; column temperature:25℃, flow rate:1.0mL·min-1; detection wavelength:203nm, injection volume:10μL. Established HPLC fingerprint of ginseng qui particles, formulated the standard fingerprint spectrum. The results show that this method is accurate and reliable, can control the whole effective participation from shenguiren granule quality.Through the experimental study, To determine the parameters normalized kernel particle prescription, preparation technology, quality standard, the drug preparation process is scientific and reasonable, stable quality, good for our body or beneficial blood and brain.
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