Dissertation > Excellent graduate degree dissertation topics show

Molecular Mechanism of Androgen Induced Transcriptional Process Regulated by Demethylases LSD1and JMJD1A

Author: WangShanShan
Tutor: WengJieMin
School: East China Normal University
Course: Biomedical
Keywords: Histone demethylation LSD1 JMJD1A androgen receptor transcriptional activity
Type: Master's thesis
Year: 2010
Downloads: 22
Quote: 0
Read: Download Dissertation


Posttranslational modifications of histone N-terminal tails such as methylation impact chromatin structure and gene transcription. Nuclear amine oxidase homolog LSD1can promote androgen-receptor-dependent transcription, and its overexpression in prostate tumors correlates significantly with relapse of the tumor during anticancer treatment. JmjC-containing demethylase JMJD1A can regulate the transcriptional activity of androgen receptor by demethylasing H3K9Me2. But their exact mechanisms as coactivators during androgen induced gene expression are not clear. To understand the molecular mechanisms of transcriptional regulation by LSD1or JMJD1A, we used lentiviral vectors carrying LSD1or JMJD1A specific shRNAs to obtain the LSD1or JMJD1A knockdown LNCaP stable cell pools. We found that knockdown of LSD1or JMJD1A decreased the expression of AR induced target genes such as PSA and NKX3.1. Chromatin immunoprecipitation assays revealed that knockdown of LSD1or JMJD1A did not significantly change the recruitment of androgen receptor, RNA polymerase Ⅱ, CBP, SRC family proteins (including SRC-1and SRC-3), and Brg1. In addition, knockdown of LSD1or JMJD1A did not affect chromatin disruption. Consistent with previous data, knockdown of LSD1or JMJD1A did affected histone demethylation of H3K9me2. Interestingly, we found that knockdown of LSD1affected the recruitment of TRAP220, while knockdown of JMJD1A did not. Our data suggested that LSD1might regulate androgen-receptor-dependent transcription through the control of mediator recruitment.

Related Dissertations

  1. Gene Mapping and Mutation Detection of a Family with Hypospadias,R695
  2. Effect of TLR4 Promoter Polymorphisms on Transcription Activity in Neonates,R346
  3. Preliminary Study on Klotho’s Transcriptional Regulation,R346
  4. The Preliminary Study of Functional C-1888T Single Nucleotide Polymorphism (SNP) Site in Promoter Region of hPLUNC Gene in Nasopharyngeal Carcinoma,R739.63
  5. Effects of ERK1/2 Signaling on the Estrogenic Activities of Genistein and Equol,R737.9
  6. The Expression and Significance of Androgen Receptor in Breast Carcinoma,R737.9
  7. Phosphorylation of AP-2α by the CK2 Kinase Results in Repression of AP-2α-mediated Transcriptional Activity,Q343
  8. The Effect of Arsenic Trioxide on Hormone-Independent Prostate Cancer PC-3 Cell Line in Vitro,R737.25
  9. Study of Expression and Transcriptional Activity of HSF1 in Human Hepatocellular Carcinoma,R735.7
  10. Study on Clonality and Loss of Heterozygosity in Pulmonary Sclerosing Hemangioma,R734.2
  11. Study on the Expression Change of ApoD and AR in the Apocrine Sweat Glands of Axillary Osmidrosis and the Role of AP-1 in it,R758.741
  12. The Expression and Trinucleotide Repeats of Androgen Receptor in Chronic Hepatitis B Virus Associated Liver Diseases,R512.62
  13. Effects of Androgen on Cardiovascular Function of Broilers,S858.31
  14. Expression of RYBP、 BMI1 and LSD1 Protein in Acute Leukemia,R733.71
  15. The Correlation Research on the Genetic Variations of Gene AR 、 SHBG and Digit Ratio with the Polycystic Ovary Syndrome (PCOS),R711.75
  16. Influence of Immunity in Mice by Sexual Hormone,S852.4
  17. The Experimental Study of Soybean Extraction on Benign Prostatic Hyperplasia,R285.5
  18. Effects of Diethylstilbestrol (DES) on the Expression of AR、ERα、Actal and PCNA Gene in Mouse Gubernacular Testis Tissue,R114
  19. Aurora B kinase inhibitor 3 - hydroxyflavone anti-tumor effect of the cum Ly-6 gene family members LYPD6 cloning and characterization analysis,Q343
  20. SIRT5 acetylation of STAT3 regulate its function,Q50
  21. Nitrosylation modification regulate NF-κB transcriptional activity of,Q504

CLC: >
© 2012 www.DissertationTopic.Net  Mobile