Dissertation > Excellent graduate degree dissertation topics show

Cardioprotective Role and Its Possible Mechanism of the Hangover Medicine on Myocardial Ischemia-reperfusion Injury in Rats

Author: ZhaoHangTian
Tutor: SunAiJun
School: Xinxiang Medical
Course: Internal Medicine
Keywords: Hangover Medicine Myocardial Ischemia Reperfusion Injury EndoplasmicReticulum Stress Aldehyde Dehydrogenase2 Cell Apoptosis
CLC: R542.2
Type: Master's thesis
Year: 2013
Downloads: 4
Quote: 0
Read: Download Dissertation


Background Myocardial ischemia-reperfusion Injury (MIRI) become the main problems of ischemic cardiomyopathy after recanalization, its occurrence and development seriously affect the prognosis of patients. Many molecular mechanism and signal pathway involved in myocardial ischemia-reperfusion injury. Oxygen free radical, calcium overload, cell apoptosis caused by variety of factors, mitochondrial membrane permeability transition pore opening and so on are involved in myocardial ischemia-reperfusion injury. And endoplasmic reticulum stress can lead to cell apoptosis, which makes the decrease in the number of live cells. Many experiments confirmed that the key enzyme of aldehyde metabolism, aldehyde dehydrogenase2(ALDH2), can play a protective role through various channels. Hangover medicine can influence ALDH2and play a protective role in development of heart failure, but views at home and abroad have not collaboration for reports of acute myocardial ischemia-reperfusion injury.Objective To investigate whether hangover medicine plays cardioprotective role in the rat model of myocardial ischemia-reperfusion injury, and to reveal the possible mechanism whether has to do with supressing endoplasmic reticulum stress responsive via on activity ALDH2. Convenient to promote the clinical treatment of myocardial ischemia-reperfusion injury to provide theoretical basis and treatment ideas.Methods200~25()g Sprague-Dawley rats were divided into3groups randomly, before myocardial ischemia and reperfusion procedure, saline (n=6), hangover medicine (n=6) or hangover medicine+cyanamide were given respectively by oral gavage and intraperitoneal injection. Calculated risk area ratio and infarction ratio by computer image analysis software. Risk area ratio=Risk area (Red ischemia area+White infarction area) /Left ventricular areax100%; Infarction ratio=White infarction area/Risk area (Red ischemia area+White infarction area)×100%. Myocardial infarction ratio was calculated by Evans blue and triphenyltetrazolium chloride (TTC) staining, and myocardial apoptosis was evaluated by TUNEL assay. Expression of endoplasmic-reticulum-stress-related proteins (Grp78,Chop) and apoptosis-related protein (Caspase12) were quantified by western-blot.Results The model were built successfully and its stability is high. The infarction size was significantly reduced by the treatment of hangover medicine (hangover medicine group vs. saline group,35.59±5.77%vs.57.72±6.52%, P<0.01). However, when the hangover medicine was blocked,the protection was diminished (hangover medicine group vs. hangover medicine and cyanamide group,35.59±5.77%vs.46.83±5.96%, P<0.01) Myocardial apoptosis and the levels of Grp78, Chop, Caspase12were not significantly different between the three groups (P>0.05).Conclusion In the early phase of myocardial ischemia and reperfusion injury, hangover medicine provides myocardial protection, which can be blocked by ALDH2inhibitor. This influence may not be implicated through ALDH2initiated endoplasmic reticulum stress

Related Dissertations

  1. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  2. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  3. The Inhibitory Effects of Celastrol on the Process of Brain Glioma Stem Cells’ Differentiation into Vascular Endothelial Cell-like Cells,R739.4
  4. Myocardial Protection Effect of L-arginine during Coronary Artery Bypass Graft Surgery,R654.2
  5. The Effects and Mechanisms of Epoxyeicosatrienoic Acids on Endoplasmic Reticulum Stress in Endothelial Cells,R363
  6. The Expression of microRNA in the Ovary of Polycystic Ovary Syndrome Rat Model,R711.75
  7. L-arginine on the Effects of Insulin Resistance and Inflammatory Factors During Cardiopulmonary Bypass,R654.1
  8. The Expression of Plk1 and Survivin in Esophageal Squamous Cell Carcinoma,R735.1
  9. Anti-tumor Activity and Safety Evaluation of Hydroxyurea Derivatives,R730.2
  10. Regulative Effect of Low-molecular Weight Heparin on the Proliferation and Apoptosis of Cultured Human Cytotrophoblast Cells under Antiendometrium Antibody Environment,R714.21
  11. Tanshinone ⅡA on Myocardial Ischemia-reperfusion Injury in Rats and Its Mechanism of Clinical Research,R285.5
  12. Protective Effect of Chlorogenic Acid on Differentiated Chondrogenic MSCs Apoptosis Induced by Hypoxia,R363
  13. Study on Expressions and Correlation of CD34、 BCL-2 in the Early Myocardial Ischemia Reperfusion Injury,R654.1
  14. Clinical Study on Cardioprotection of Nicorandil Preconditioning Against Ischemia/Reperfusion Injury in Patients Underwent Double Valve Replacement,R654.2
  15. Captopril and Losartan on Angiotensin Ⅱ-induced rat vascular smooth muscle cells Wista bcl-2 and Fas gene expression inhibition,R965
  16. Effect of Agkistrodon Halys Pallas Venom on the Proliferation and Apoptosis of Cultured Human Synovial Cells,R285.5
  17. The Apoptosis of Lewis Lung Carcinoma Cell Induced by 18F-2-Deoxy-2-Fluoro-D-Glucose,R734.2
  18. Effect of Combining Breviscapine with Ischemic /Preconditioning on the Expression of Myocardial Protein TNF-α、NF-κB during Myocardial Ischemia/Reperfusion in Rabbits,R541
  19. Ganglioside GD1a Induces FBJ-cell Apoptosis under Serum-free Conditions,R341
  20. Study on the Hydrolysis and Absorption of Glutaminate-containing Dipeptides and Their Effects on the Jejunum Development of Weaned Piglets,S828.5
  21. Role of JAK2-STAT3 Pathway in Cardioprotective Effect of Bradykinin Postconditioning,R541

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases
© 2012 www.DissertationTopic.Net  Mobile