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Expression of Netrin-1Protein in Early Diabetic Retinopathy

Author: ZhaoZuo
Tutor: PengXiaoYan
School: Capital University of Medical Sciences
Course: Ophthalmology
Keywords: Netrin-1 early diabetic retinopathy Diabetic microangiopathy Nuerodegeneration
CLC: R774.1
Type: Master's thesis
Year: 2013
Downloads: 8
Quote: 0
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Abstract


Objective To observe the expression of netrin-1protein in retina of STZ induceddiabetic mice,evaluate the relativity between netrin-1and the early stage of diabeticretinopathy.Methods To select60male C57BL/6mice of good health,distinguish them to be3group,45in diabetic group and15in control group.Establish STZ-induced diabeticmice model by intraperitoneal injection of streptozocin(STZ);Measure the bloodsugar and weight in different stage of diabetes,HE stain will be used to observe thepathology change of early diabetic retinopathy,use immunofluorescence to observethe expression of netrin-1protein in different times of diabetic retinopathy.Results The blood sugar of diabetic group is much higher than control in4,8and12weeks after injection;in diabetic group,the blood capillary vessel wall isthick,limiting layer get to be thick,ganglion cell engorge and disorder,and there ismicroangioma growing to cavum vitreum.4,8and12weeks after injection.Theexpression of netrin-1protein is mainly expressed in GCL and INL.There is noexpression of netrin-1protein in WESTERN-BLOT.Conclusion STZ-induced diabetic mice model is suitable for the study of earlydiabetic retinopathy.The pathology change of retina is manifest,and there ismicroangioma.The expression of netrin-1protein in diabetic group is higher thancontrol group,which is the same as the expression of Netrin-1mRNA. Regulation ofnetrin-1in angiogenesis and axon guidance offers new hope in pathogenesy researchand clinical treatment of correlated disease.

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CLC: > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease
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