Dissertation > Excellent graduate degree dissertation topics show

Experimental study of vitamin A deficiency on fetal rat Hoxd3 gene and the expression of TGF- β 1 Effect

Author: LiuChuYin
Tutor: TanMingSheng
School: Beijing University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Vitamin A deficiency Hoxd3gene TGF-β1 RARs
CLC: R591.4
Type: Master's thesis
Year: 2014
Downloads: 0
Quote: 0
Read: Download Dissertation

Abstract


Vitamin A plays an important role in the growth and development of animals, which mainly generated by in vivo metabolite retinoic acid, especially in the embryonic stage of development. The regulation effects of Vitamin A on the development of the spine mainly by regulating the expression of Hox genes. Both of exogenous RA intervention or endogenous RA inactivation can also lead to abnormal Hox gene expression. Decreased levels of vitamin A pregnant mother can affect the growth and development of the offspring, which often leads to deformities Spinal deformities, including the craniovertebral junction de-formity. Studies have shown that Hox gene family gene-Hoxd3is the most important regulative gene in development of craniovertebral junction, and its lack of expression often induce craniovertebral junction deformity. However, vitamin A deficiency affecting normal embryonic development process is very complex, there are other regulatory mechanisms. Modern medical studies have shown that vitamin A and transforming growth factor-β has a close relationship during embryonic development, for the development of various tissues and organs of suppression or synergy with each other. TGF-β is a family of polypeptides with multiple functions, widely present in normal cells and transformed animal cells, which is most rich in the platelets and bone structure. TGF-β1as the significant member of the TGF-βs family, promote cell proliferation, cell differentiation, and extracellular matrix synthesis. More importantly, it could promote growth and differentiation of mesenchymal cells. therefore Vitamin A deficiency may lead to decreased TGF-β1expression in fetal, resulting in decrease in osteoblast differentiation, growth retardation of skeleton, craniovertebral junction deformities, and congenital skeleton mal-forma-tions.Objective:This paper is divided into literature review and experimental study in two parts. Literature review describes the interaction between the main role of vitamin A in embryonic development and the interaction with TGF-β and the regulation of Hox gene expression. Also it explains the mechanism of Hox gene that causes spinal deformity. Experimental study focused on vitamin A deficiency leads to reduced gene expression Hoxd3mechanism causing crani-overtebral junction deformities, and a lack of vitamin A in the process leading to craniovertebral junction deformities with TGF-β1possible correlation.Methods:36female C57BL/6mice were divided into2groups, namely normal control group (N), vitamin A deficiency group(VAD). N group were fed with normal diet, while VAD group were fed with vitamin A deficiency diet. After modeling success, half of mice had been cesareans and its embryos were taken for detecting the expression levels of Hoxd3gene AND retinoic acid receptors mRNA by RT-QPCR On11.5st gestational day. The rest of pregnant mice had been cesareans and its embryos were taken for detecting the level of TGF-β1by immunohistochemistry On21st gestational day.Result:(1)The expression levels of Hoxd3gene and RARs mRNA and were significantly lower in VAD group than N group (P<0.01).(2) There was significant difference in TGF-β1between N group and VAD group in craniovertebral junction (P<0.01).Conclusion:Vitamin A deficiency could decrease the expression level of Hoxd3gene related to depression of RARs during embryo development, which could induce congenital malformations of the craniovertebral junction. And we also found that Vitamin A deficiency could decrease the expression level of TGF-β1in bone mass of raniovertebral junction, which might indicate that the interaction between Vitamin A and TGF-β1in pattern of craniovertebral junction.

Related Dissertations

  1. Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats,R285.5
  2. Salt-induced Vascular Remodeling and Telmisartan Intervention in Wistar Rats,R544.1
  3. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  4. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9
  5. h-VEGF165 Genetransfer Promoted the Tissue Repair of Maxillofacial Region in Rat,R730.5
  6. Zun Shen Zhuang Gu Tang（尊肾壮骨汤） Affect Transformation Growth Factor Influence of TGF-β1 Expression in Bone Repair,R274.1
  7. Experimental Study on the Mechanism of the Induction of TGF-β1 in the Synthesis of Extracellular Matrix in Mdscs.,R329
  8. Impact of Thoracic X-ray Irradiation on Foxp3 and TGF-β1 Expression in Lung Tissue and Its Clinical Significance,R818
  9. RAS dysfunction induced by high glucose in renal tubular epithelial cells and its mechanism of action,R692.9
  10. Effects of Endostar on Immune State of Lung Metastasis of Melanoma B16 Cells in C57BL/6 Mice,R739.5
  11. Vitamin A deficiency on lung morphogenesis of comparative study,R714.2
  12. HTRA3 in Rat Ovary carcinoma and its impact on follicular development,R711.75
  13. Silicosis and pulmonary fibrosis in rats during the critical difference in gene expression and signal transduction pathways Discussion,R563.9
  14. RGDS peptide on collagen deposition in airways of asthmatic rats and IL-13 and TGF-β1 expression,R562.25
  15. Vitamin D deficiency in pregnant rats offspring of long bones vitamin D receptor and bone development in gene expression,R714.2
  16. Effect of Morphology and Express of Platelet-derived Growth Factor-A of Mild Vitamin A Deficiency on Rat Lung Development,R591.1
  17. The Experiment to Improve Effects of Vitamin A Deficiency and Preliminary Study of Vitamin A Product,R151
  18. The Expression and Significance of TGF-β1 and CyclinD1 in Parathyroid Tumors,R736.2
  19. The Expression and Significance of CD4、CD25、Foxp3 and ERα、TGF-β1 in Autoimmune Liver Disease,R575
  20. Role of UGBP in Antiflammin-1 Anti-proliferation of Lung Fibroblasts and Primary Mechanism,R329
  21. Effects of TGF-β1 on RT1-A and RT1-DM Expression in Uterus and Placenta in Different Phases of Mouse Pregnancy,Q954.4

CLC: > Medicine, health > Internal Medicine > Systemic disease > Nutritional deficiency > Vitamin deficiency
© 2012 www.DissertationTopic.Net  Mobile