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The Expression of YKL-40in the Hippocampus of Rats after Status Epilepticus

Author: HuHui
Tutor: LiShuYu
School: Central South University
Course: Clinical
Keywords: status epilepticus pilocarpine hippocampus YKL-40 inflammation
CLC: R742.1
Type: Master's thesis
Year: 2013
Downloads: 9
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Abstract


Object:To investigate the expression of cartilage glycoprotein-39(YKL-40) in the hippocampus following status epilepticus in rats and to explore the possible mechanism of YKL-40in SE-induced neuronal injury.Methods:healthy adult male Sprague-Dawley rats were randomly assigned to control group and SE model group. The Lithium chloride-pilocarpine SE model was established, and the control group were injected with Sodium Chloride. The hippocampus was chosen for study at3h、6h、12h、24h and72h after SE. YKL-40protein was detected by immunohistochemical; double immunofluorescence was used to observe the cellular expression of YKL-40/NeuN; YKL-40mRNA was examined via real-time quantitative PCR.Results:1. expression of YKL-40protein:YKL-40immunoreactivity in control group was poor that mainly localized in the cytoplasm within the neuron of hippocampus CA3/CA4, little in nucleus; YKL-40immunoreactivity exhibited a diffuse distribution in the whole cytoplasm within the neuron after SE, some in nucleus. Compared with the control group, the expression of CA3YKL-40protein in SE group was increased gradually at6h(P<0.01), and reached a peak at12h (P<0.001), then decreased at24h(P>0.05), By72h however, YKL-40expression levels had nearly returned to control levels; the expression of CA4YKL-40protein in SE group was increased at6h, but no statistical significance (P>0.05), then reached a peak at12h (P<0.01), decreased at 24h(P>0.01), at72h no statistical significance. Double immunofluorescence about YKL-40/NeuN was revealed that YKL-40was located in the neuron of hippocampus CA3/CA4.2. expression of YKL-40mRNA:SE induced a rapid increase of YKL-40mRNA beginning at6h, peaked at12h(P<0.01),3h、24h and72h were no statistically significant difference compared with control group.Conclusions:The increased expression of YKL-40in SE rat hippocampus may involve in the pathogenesis of neuron injury following SE.Figure12, table5,61references.

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CLC: > Medicine, health > Neurology and psychiatry > Neurology > Brain diseases > Epilepsy
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