Dissertation > Excellent graduate degree dissertation topics show

Biological Effects of PCDH8Gene Expression in Nasopharyngeal Carcinoma Cell

Author: ShiYuLiang
Tutor: HuGuoHua
School: Chongqing Medical University
Course: Otorhinolaryngology
Keywords: PCDH8 nasopharyngeal carcinoma CNE-1
CLC: R739.63
Type: Master's thesis
Year: 2013
Downloads: 17
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigation the biological effects of PCDH8gene onthe proliferation, the cycle,the invasion, the chemosensitivity test andtumor genesis ability in nasopharyngeal carcinoma cell line CNE-1aftertransfected pcDNA3.1-PCDH8and pcDNA3.1plasmind.Methods: The eukaryotic expression plasmidpcDNA3.1-PCDH8(experimental group) and pcDNA3.1(control group)were transfected into CNE-1cells respectively with lipofectamine, andestablished the stable PCDH8expression cancer cell line. RT-PCR andWestern blot methods were used to examined the expression of PCDH8mRNA and protein in CNE-1cells after transfected.CCK-8assay, FCM,transwell invasion assay，chemosensitivity assay were used to study theeffects of PCDH8expression on the proliferation, the cycle, the invasion,chemosensitivity in CNE-1cells. Establish nasopharygeal carcinomatransphlanted tumor model in nude mice to obverse the effect on the growthof transplanted tumor. The mRNA level of MMP-9, MMP-2, VEGF weremeasured by real time PCR. The protein level of CDK-4, P21, P27, JNKwere measured by western blot.Results:①RT-PCR and Western-blot showed that the PCDH8experssion was higher in cells transfected with pcDNA3.1-PCDH8than inthose transfected pcDNA3.1(P<0.05).②The cellgrowth assay wasanalysed used CCK-8test and showed a significant reduction inexperimental group cells compared with control group(P<0.05).③The FCM showed that the PCDH8expression caused a significant accumulationof cells in the G0/G1phase compared with control group cells,and aconcomitant decrease of experimental group cells in the S phase comparedwith control group cells.(P<0.05).④Transwell matrigel invasion assayrevealed a obvious reduction in the invasive ability of the experimentalgroup cells compared with the control group cells(P<0.05).⑤Thechemosensitivity assay showed the chemosensitivity to cis-platinum ofexperimental cells significantly enhance(P<0.05).⑥The animal model ofNPC was established successfully.The tumor of experimental group wasgrew slower than the control group.⑦Compared with the control group,the experimental group of MMP-9mRNA level by the Real time PCR washigher, but the differences of the expression of MMP-2,VEGF mRNA werenot singnificant.⑧Compared with the control group, the experimental ofCDK-4,P27,JNK protein level by western blot was higher. The differencesof P21was not singnificant.Conclusion: The pcDNA3.1-PCDH8and pcDNA3.1plasmidsuccessfully transfected into nasopharyngeal carcinoma cell line CNE-1bylipofectamine.The PCDH8eukaryotic expression can decrease theproliferation, invasion ability, slow down the cycle, increase the Cis-DDPsensitivity ability and inhibit the growth of transplanted tumor.PCDH8maybe involved in the incidence and development of NPC through MMP-9，CDK-4,P27,JNK gene or related signal. The PCDH8as a candidate tumorsuppressor for nasopharyngeal carcinoma.

Related Dissertations

  1. Research for the Expressive Correlation of EBERs and BMI-1 in Nasopharyngeal Carcinoma,R739.63
  2. Related Risk Factors to Caries after Nasopharyngeal Carcinoma Radiotherapy,R739.63
  3. The Antitumor Effect of Different Radiation Pattern and Cetuximab in CNE-2 Xenografted and the Expression of Bax、Bcl-2,R739.6
  4. 临床研究 Neo-adjuvant Docetaxel and Cisplatin Followed by Concurrent Docetaxel and Cisplatin with Intensity-modulated Radiation Therapy in Treatment of Locally Advanced Recurrent Nasopharynx Cancer,R739.63
  5. The Different Effects of MicroRNA on the Same Target Gene in Normal and Hypoxia Condition of CNE,Q343
  6. LMP-1 to Human Nasopharyngeal Carcinoma Cell Line CNE1 MiRNA Expression Spectrum Influence,R739.63
  7. The Study of Correlations between EB Virus Infection and Survivin Expression in Han and Uyghur Nasopharyngeal Carcinoma in Xin Jiang,R739.63
  8. The Induction of Apoptosis in Gastric Cancer Cell Line SGC-7901 and Nasopharynageal Cancer Cell Line CNE by ZTO,R735.2
  9. A Study on the Effect and Mechanism of Artemisinin and Artemether on the Radiosensitivity of Human CNE-1 Nasopharyngeal Carcinoma Cell,R739.63
  10. Effect of Different Radiation Pattern on Cell Cycle and Cyclins in Human Poor Differential Nasopharyngeal Squamous Cell Carcinoma,R739.63
  11. To Detect the Expression of Ku80、ATM by Simulational Intensity Modulated Radiation Therapy Model in Human Poor Differential Nasopharyngeal Squamous Cell Carcinoma,R739.63
  12. Study on 5-ALA Photodynamic Effect to CNE Cell Line,R739.63
  13. Research on the Migratory and Invasive Effects of CNE Line after ALA Photodynamic Therapy,R965
  14. The Phase Distribution of Cell Cycle in Parental and Taxol-resistant Nasopharyngeal Carcinoma,R739.63
  15. Expression and clinic significance of cox-2 protein in nasopharyngeal carcinoma,R739.63
  16. Study of Effect of Meloxicam on CNE-1 Cell Lines in Nasopharygeal Carcinoma,R739.63
  17. A Research Investigating the Tumor Inhibition Rate, Expression of Cyclin D1 and B1 on the CNE-1 Xenografted Nude Mice in Intensity-modulated Radiationtherapy Mode,R734.2
  18. Improved Cne PRP8 by Directed Evolution,TQ463.6
  19. The Study of Radiation Wound on Nasal Mucosa of Patients with Nasopharyngeal Carcinoma,R739.63
  20. Recurrent nasopharyngeal IMRT program design,R739.63
  21. Construction, Expression and Function of Two Eukaryotic Expression Vectors with gp350 Gene and gp350-C3d3 Fusion Gene,R346

CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
© 2012 www.DissertationTopic.Net  Mobile