Dissertation > Excellent graduate degree dissertation topics show

The Expression and Significance of Toll-like Receptor2,3,4on Head and Neck Squamous Cells(HNSCs)

Author: WangZuoJing
Tutor: WangJunFu
School: Jinan University
Course: Immunology
Keywords: TLR Laryngocarcinoma carcinoma hypopharyngeal carcinoma Western blot RT-PCR immunohistochemistry
CLC: R739.91
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation

Abstract


ObjectsLaryngocarcinoma or hypopharyngeal carcinoma, the most common pathological type ofwhich is the squamous cell carcinoma, shows high degree of malignancy account for1-5%of themalignant tumor and is often with lymph node metastases around the neck. Surgery operation isthe main therapeutic method for this severe disease. Throat is often in a inflammation situationbecause of direct contact with environment which leads to physical or chemical stimulation.Chronic inflammation plays a significant role in the process of tumorgenesis.Toll-like receptor is an important pattern recognition receptor that recognizes highconservative molecular structure, pathogen-associated molecular patterns and danger-associatedmolecular patterns. TLRs is a kind of classic transmembrane protein. As one of the mostcharacteristic PPR molecule, it can recruit several adaptor protein and trigger signal transduction,activate the specific transcription factors, lead to antibacterial peptide, pro-inflammatorycytokines and chemokines,antiviral agent, adhesion molecule and other active factors.Research results demonstrate that TLRs expresses highly not only in immune cells, but alsoin many kinds of malignancy cells, especially in inflammatory related tumors. It can exert animportant effect in the process of tumor and treatment. This research will study the expressionintensity of TLR2,3and4at transcription and protein levels, using their correspondingpara-carcinoma tissue and polyp tissue as control. On the basis of our results, to discuss the influence of TLR2,3and4in cancer process of Laryngocarcinoma or hypopharyngealcarcinoma refering to other findings. We hope to offer more ideas about cancer dignosis,prevention and treatment.Methods and results1．Detecting the expression levels of TLR2,3,4mRNA in diffrent type pathological tissue byRT-PCR.1.1The diffrent expression level of TLR2,3,4mRNA in tumor tissue and vocal polyps.TLR2,3,4mRNA expressed in tumor tissue, but fairly expressed in the vocal polyps. TLR4isthe top one, followed by TLR2. TLRs expression level in tumor tissue was stronger than that inthe vocal polyps. TLR2t=6.723，TLR3t=4.976, TLR4t=7.645，all P<0.01. This statistic resulthas great significance.1.2The diffrent expression levels of TLR2,3,4mRNA in tumor tissue and its correspondingadjacent tissue of cancer.TLR2,3,4mRNA expressed both in tumor and the adjacent tissue of cancer. Their expressionlevels in tumor tissue was stronger than that in the adjacent tissue of tumor. TLR2t=6.179，TLR3t=4.831, TLR4t=6.057，all P<0.01. This statistic result has great significance.1.3The diffrent expression levels of TLR2,3,4mRNA in different tumor stages.TLR2,3,4mRNA have great significance in different tumor clinical and TNM stages and theirexpression levels were closely associated with the tumor stages. ANOVA results: in differenttumor clinical stages: TLR2F=5.150，TLR3F=3.700，TLR4F=4.862，all P <0.05. TNM(N)stage：TLR2F=5.562，TLR3F=7.200，TLR4F=15.039，all P <0.05. All of these statisticresults have great significance.1.4The diffrent expression levels of TLR2,3,4mRNA in different pathological grade of cancer.The expression levels of TLR2,4mRNA is positive associated with its grade malignancy,while the expression intensity of TLR3mRNA may has not significance with it in cancer tissue. 2．Detecting the expression levels of TLR2,4protein in diffrent types of pathological tissue byWesteron blot.2.1The diffrent expression levels of TLR2,4protein in cancer tissue and the vocal polyps.Protein expression levels of TLR2,4in tumor tissue were stronger than that in the vocalpolyps. Especially that TLR2showed the more difference between in these two kinds of tissue.2.2The diffrent expression levels of TLR2、4proteins in cancer tissue and the adjacent tissueof cancer.Protein expression levels of TLR2,4in cancer tissue were stronger than that in the adjacenttissue of cancer.2.3The diffrent expression levels of TLR2,4protein in different tumor stages.TLR2、4protein have great significance in different tumor clinical and TNM stages and theirexpression levels were closely associated with the tumor stages.2.4The diffrent expression levels of TLR2,4protein in different pathological grade of cancer.Threre were positive correlation between protein expression levels of TLR2,4and themalignant degree of tumor.3. Detecting the expression locations of TLR2,4protein in the squamous carcinoma cells byimmunohistochemistry.Both TLR2,4expressed on cell membrane and they also can be seen in cytoplasm of thesquamous carcinoma cells. Anti-TLR2,4Ab，positive cells showing cytoplasmic and membranebound reaction.Conclusion: TLR2,3,4expressed usually in the Laryngocarcinoma or hypopharyngealcarcinoma tissue but fairly seen in the vocal polyps. Both TLR2,4expressed on cell membraneand they also can be seen in cytoplasm of the squamous carcinoma cells. Expression levels ofTLR2,3,4mRNA or protein showed a great significance between in the cancer tissue and theircorresponding adjacent tissue of cancer. The expression levels of TLR2,4mRNA is positiveassociated with its grade malignancy, while the expression intensity of TLR3mRNA might has not significance with it in cancer tissue.Significance of researchThis project confirmed the expression levels of TLR2、3and4mRNA and protein levels ofTLR2and4as well as cellular localization in Laryngocarcinoma or hypopharyngeal carcinomatissue. We also make sure the relation about expression intensity of these TLRs with tumor stagesand lymph node metastasis. This can offer theory evidence for the cilinic application.

Related Dissertations

  1. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  2. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  3. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  4. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  5. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  6. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  7. Molecular Mapping of Downy Mildew Resistant Gene and Expression of Rs-AFPs in Radish (Raphanus sativus L.),S631.1
  8. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  9. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  10. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  11. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  12. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  13. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  14. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  15. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  16. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  17. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  18. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  19. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  20. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  21. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31

CLC: > Medicine, health > Oncology > Other parts of the tumor > Head, neck, shoulder tumor
© 2012 www.DissertationTopic.Net  Mobile