Dissertation > Excellent graduate degree dissertation topics show

Involvement of the PI3K/Akt Signaling Pathway in Platelet-Derived Growth Factor-Induced Migration of Human Lens Epithelial Cells

Author: XiongZuo
Tutor: TangLuoSheng
School: Central South University
Course: Clinical
Keywords: Posterior capsular opacification PDGF migration oflens epithelial cell PI3K/Akt pathway
CLC: R776.1
Type: PhD thesis
Year: 2012
Downloads: 28
Quote: 0
Read: Download Dissertation

Abstract


PURPOSE Posterior capsular opacification (PCO) is caused partially by the migration of lens epithelial cells. To date, the mechanism of the migration is largely unknown. The purpose of this study was to investigate the effect of platelet-derived growth factor (PDGF)-triggered signaling pathways and its downstream effectors in the migration of lens epithelial cells.METHODS In vitro scratch-wound healing and transwell migration assays were used to measure the migration of lens epithelial cells. The activation of PDGFRβ, phosphatidylinositol3-kinase (PI3K)/protein kinase B (Akt) and mitogen activation protein kinase (MAPK) pathways, the impact of PDGF treatment on the expression of cell protrusion molecules, and the stabilization of β-catenin were measured by western blotting. The translocation of β-catenin was detected using indirect immunofluorescence.RESULTS PDGF was found to enhance cell migration, which depended on the PI3K/Akt pathway. The activation of the PI3K/Akt pathway by the PDGF/PDGFRβ axis induced the upregulation of cell protrusion molecules and stabilization and translocation of β-catenin, contributing to enhanced cell migration. CONCLUSION Data from this study directly linked the central PI3K/Akt pathway to lens epithelial cell migration and pointed to new avenues for therapeutic intervention in PCO.

Related Dissertations

  1. Effects of ROCKⅠon Migration of Vascular Smooth Muscle Cells Induced by PDGF,R363
  2. Down-regulation of ROCK Isoforms on Migration and Proliferation of Vascular Smooth Muscle Cells Stimulated by PDGF,R363
  3. Inhibitory Effect of Ginsenoside Rb1 on Proliferation of Vascular Smooth Muscle Cells and Carotid Neointimal Hyperplasia Induced by Balloon Injury,R285.5
  4. Effects of Total Flavonoids of Drynaria on Proliferation and Osteogenic Differentiation of Rat Dental Pulp Stem Cells,R285.5
  5. Clinicopathological Findings and Expression of Glut-1 and PI3K/Akt Signal Pathway in Adenoid Cystic Carcinomas,R739.8
  6. Content Analysis of Serum VEGF and PDGF in the Patients of Fructure Complicated with Traumatic Brain Injury,R651.1
  7. New bio- sensor technology for the detection of nucleic acids and proteins,Q52
  8. Preliminary Study on the Anti-apoptosis Mechanism Via PI3K/Akt Signal Pathway Induced by GABA_B Receptor in Cerebella Granule Neurons,Q42
  9. Expression and Clinical Significance of PDGF-B in Non-small Cell Lung Cancer and Relationship with Microvessel Density,R734.2
  10. Gene Expression Profiles, Proliferation and Extracellular Matrix Production of hUCMSCs in Response to TGF-β1,R363
  11. Molecular Biological Mechanism Study on Chronic Renal Failure Model in Rats with Shenqi Jiedu Decoction,R285.5
  12. Effects of Oligofectamine Mediated PDGF-Α Receptor Asodn Transfection on Rpe Cell Proliferation and Apoptosis in Vitro,R774.1
  13. T-cell non-Hodgkin 's lymphoma disseminated chemokine receptors experimental study,R733.1
  14. Effects of Baicalin on Rabbit Lens Epithelial Cell Proliferation Inhibition and NF-κB,R285.5
  15. Capsular bag injection of 5 - fluorouracil suppression of posterior capsular opacification clinical research,R779.66
  16. PI3K/Akt Path Way of Rat Cerebral Endothelial Cells Activated by Hypoxia and Regulation Neurovascular Unit,R285.5
  17. The Study of Delayed Wound Healing in Mice with Combined Radiation and Wound Injury,R818
  18. Bortezomib on acute myeloid leukemia cells SHI_1 Influence of PI3K/Akt pathway,R733.7
  19. Inhibitory Effect of Low Molecular Weight Heparin on Posterior Capsular Opacification and the Concentration of PDGF、EGF in Aqueous Humor of the Rabbit,R776.1
  20. The Effect of Prevention of Plasmin on Posterior Capsule Opacity,R779.66
  21. The Molecular Mechanism of Celecoxib Regulating Apoptosis in Gastric Cancer Cells Via PI3K/Akt Pathway,R735.2

CLC: > Medicine, health > Ophthalmology > Lens and vitreous diseases > Cataract
© 2012 www.DissertationTopic.Net  Mobile