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Observation of Er:YAG Laser Lattice Therapy Skin Tendering on Animal Experiments

Author: SuMingZuo
Tutor: JiaYuJie
School: Dalian Medical University
Course: Pathology and Pathophysiology
Keywords: Er YAG laser lattice collagen fibers elasticity fibers thickness of innerskin
CLC: R751.05
Type: Master's thesis
Year: 2012
Downloads: 7
Quote: 0
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Abstract


Aims:1) After having researched for irradiating with Er;YAG laser lattice continuously on the naked back skin of Kun Ming mice, what is the change of inner skin thickness, collagen fibers and elasticity fibers.2) Doing the research about the influence on skin thickness, collagen fiber and elasticity fiber after increasing irradiative energy.Methods:Take out108male (clean level) white Kun Ming mice randomly at the age of eight to ten and the weight are about seventeen to twenty-three grams, which are divided into three groups (high-energy group, low-energy group and comparison group), continuously irradiating Er:YAG lattice on the naked back skin of Kun Ming mice at two different levels (100J/cm2,25J/cm2). After irradiating for one day, three days, seven days, fourteen days, twenty-eight days and fifty-six days, there will be a research on measuring the changes of back skin thickness and the number of elasticity fibers which are special stained between experiment groups and comparison group.Results:1) At first, there is a series of slightly inseparable dotted harm after irradiating Er:YAG lattice on the naked back skin of Kun Ming mice. And then irradiating Er:YAG lattice making skin to become red and systemic inflammatory reaction appeared for one to two days, inflammation and necrosis formed slough then shed off step by step for three to four days, making slough shed off completely for six days. Finally, parts of collagen fibers reshaped after seven days to fifty-six days. Obviously, inner skin begins to be thickness after irradiation for fourteen days, besides that, collagen fibers and elasticity fibers keep on proliferation and will be higher than comparison group till fifty-six days.2) High-energy group and low-energy group make proliferation on collagen fibers and elasticity fibers obviously shows that increasing energy will strengthen cure purpose.Results:Irradiating Er:YAG lattice on the back skin of Kun Ming mice will stimulate inner skin collagen fibers and elasticity fibers proliferated and make inner skin thicken. In a certain range, the thickness of inner skin and the number of proliferation of elasticity fibers will be increased with increasing energy.

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CLC: > Medicine, health > Dermatology and Venereology > Dermatology > Skin disease therapy
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