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Preparation and skin penetration properties of collagen liposome

Author: ZuoSen
Tutor: FanJun
School: Northwestern University
Course: Pharmaceutical Engineering
Keywords: collagen protein liposomes through skin film scattered micro-column centrifugation
CLC: R944.1
Type: Master's thesis
Year: 2012
Downloads: 192
Quote: 0
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Abstract


Protein drugs through in vitro absorption way to enter circulation biological drugs is an important direction, combined with physiology, natural medicinal chemistry pharmacyokine-tics, and other disciplines related technology. Collagen protein to through in vitro through skin into true cortex function in leather fibre, will penetrate corneous layer and the skin surface layer of the skin membrane. Due to the collagen protein itself molecular weight can achieve is water soluble substances and tens of thousands of Dalton, so make collagen protein in biological activity, don’t lose the premise of minimum molecular weight and make it close oil properties, is to improve the utilization ratio of through skin an important research direction. We use the present study is widely used, attention is high drug carriers technology to find a new way to solve the problem.High Performance Liquid Chromatography collagen protein content, the investigation of the liposome of collagen uptake through skin and absorption rate. The study by high performance liquid chromatography (HPLC) method to detect the molecular weight of less than5K Da collagen protein content of the method, think for chromatographic column chromatography conditions:ZORBAX SB-using C18,4.6x250mm (5μl), mobile phase:A is to contain5%acetonitrile and0.05%aqueous solution TFA, B for acetonitrile. Detected wavelength for220nm; Flow rate:0.1ml/min; The column temperature35℃. In order to improve the collagen in vitro absorbed through skin performance, with lecithin and cholesterol for film materials, film for fish skin scattered legal system of liposome hydrolyzed collagen, the characterization, liposomes particle size for130+10nm, the encapsulation efficiency may reach above60%,3.1%of the drug. The simulation Franze diffusion pool for mice from the body skin penetration diffusion tests showed that the molecular weight of3500Da fishskin hydrolyzed collagen the liposome after sealing bag, can absorb through skin in24h can reach21.9%, more direct daub collagen through skin utilization doubled. Proof liposome of small molecules collagen can improve the i through skin absorbed performance.

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