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Myosin X was Involved in NLT Cell Migration and Cell Polarity

Author: GuoYuGuang
Tutor: ZhuZuoJuan
School: Northeast Normal University
Course: Cell Biology
Keywords: Cell migration Cell polarity N-Cadherin Myo Ⅹ
CLC: Q42
Type: Master's thesis
Year: 2010
Downloads: 40
Quote: 0
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Abstract


Myosin Ⅹ (Myosin X, Myo of X) which only non-traditional expression of myosin in vertebrates, it exercise its mission in the interaction of actin filaments, microtubules, which suggests that there is a link of Myo X and cell migration. The existing laboratory results in Myo X play a role in the adhesion between cells and the extracellular matrix and cell migration. N-cadherin is one of the important members of the cadherin family of transmembrane glycoproteins, existing research results show that N-cadherin and cytoskeletal microtubule interactions in the low expression of E-Cadherin cells transfected with N-Cadherin can increase cell migration, improve the efficiency of cell adhesion, suggesting that N-Cadherin can promote cell migration and invasion. N-Cadherin is controlled by adjusting the relationship between the cytoskeleton and the extracellular matrix and centrioles, and location of the nucleus and the cell polarity. And N-cadherin activity can weaken the directional migration of cells, the formation of filopodia, polarity, and in wound healing in the Golgi redirect. We applied RNA interference technology to reduce the endogenous Myo Ⅹ NLT cells express, first of all we through Transwell analysis found, expression Scramble miRNA and Myo Ⅹ miRNA of NLT cells directed significant difference in the migration effect, significantly reduced the number of Myo Ⅹ miRNA cell migration, Myo Ⅹ miRNA lead shorten the distance migrated by the migration ability of the cells is reduced; collagen gel analysis under the same experimental conditions, the transfection of Myo X miRNA NLT cells, and cell migration loss of directionality; analysis of Wound Healing Wound healing, immunofluorescence staining of the Golgi marker detection of cell polarity, the results showed that transfection of Myo X miRNA cell polarity change, loss of polarity characteristics of the serum-induced Golgi arranged toward the wound; the immunofluorescence staining mark Myo Ⅹ and N-Cadherin two proteins, NLT cells co-localized phenomenon the silence of Myo X N-cadherin expression was reduced, the Western blot results further validate the N-cadherin expression of endogenous Myo Ⅹ related. This study shows that to reduce in Myo X reduced expression in NLT cells decreased cell migration ability to influence the direction of cell migration, and to N-cadherin expression down. This study is a further advantage of the explant culture and the use of GnRH transgenic mice in Myo X GnRH neuronal migration process laid the foundation.

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