Dissertation > Excellent graduate degree dissertation topics show

Study on Attachment of Leptospirllum Ferriphilum YSK Grown on Different Energy Sources to Pyrite Surface and Surface Properties

Author: SuLiJun
Tutor: ZhouHongBo；GuZuoHua
School: Central South University
Course: Microbiology
Keywords: Pyrite To addicted cleek end pylori Adsorption Real - time quantitative PCR Surface properties
CLC: Q93
Type: Master's thesis
Year: 2010
Downloads: 79
Quote: 0
Read: Download Dissertation

Abstract

Addicted iron hook end pylori (Leptospirillum ferriphilum) Gram-negative bacteria, the environment, can only be obtained by the oxidation of ferrous ion energy can be grown in extreme acidic (pH1.5-1.8), is an obligate aerobic microorganisms. In the the bioleaching study, addicted iron hook end pylori has been widely reported, and is considered to be the main iron-oxidizing bacteria. Pyrite is the most widely distributed sulfide ore, often colored with copper, molybdenum, gold, silver, cobalt, gallium, rare metals the elements associated or symbiotic. In order to investigate the mechanism of bacterial leaching of pyrite, improve the efficiency of the bioleaching industrial practice, different energy culture addicted to iron hook end pylori study investigated the growth characteristics change and leaching of pyrite effects; using quantitative real-time PCR method was measured under different conditions in the pyrite surface adsorption; through the amount of adsorption, infrared spectroscopy, zeta potential and contact angle measurement, and atomic force microscopy surface characterization, study of pyrite with bacteria the rear surface of the changing nature of the role, and laid a foundation for revealing the mechanism of action of microorganisms and pyrite. By to draw L. ferriphilum YSK growth curves in different energy substances (FeSO4 · 7H2O and pyrite), that for the energy growth of L. ferriphilum YSK than pyrite to ferrous energy growth of bacteria growth cycle long. Ferrous cultured bacteria will soon be able to enter the logarithmic growth phase, the generation of 22.1h; pyrite cultured bacteria enter a longer time on the logarithmic growth phase for 12h so generation 33.2h than to ferrous Energy cultured bacteria about 11 hours long. Shake flask leaching of pyrite experimental studies have shown that the pyrite leaching is a process of acid production, the pH of the leaching process is a downward trend, while the redox potential of rising early in the leaching, the formation of the high redox potential conducive to the leaching of pyrite. L. ferriphilum YSK pyrite culture than to the ferrous energy growth of the bacteria can adapt to the the leaching system's environment, and the leaching effect compared to a lot better. Late leaching solution accumulated a higher concentration of Fe3 generate the jarosite hindered the dissolution of pyrite, pulp potential downward trend, leading to slower leaching rate. The adsorption behavior of the application of real-time quantitative PCR studies of different energy training the bioleaching bacteria L.ferriphilumYSK pyrite is proved to be feasible, the results show that different energy culture L. ferriphilum obvious pyrite surface adsorption phenomenon, from 30 minutes to 40 minutes between both reach equilibrium, high but pyrite cultured L.ferriphilum soluble ferrous ions in the the pyrite surface adsorption than culture. Played a crucial role in the adsorption process, the surface properties of the bacteria. Changes of the pH of the solution makes the surface properties of the bacteria has changed, thus affecting the adsorption behavior of the bacteria in the mineral surface, the pH value is affecting bioleaching bacteria L. ferriphilum YSK pyrite surface adsorption of the more critical factors, The adsorption amount under acidic conditions; temperature on bioleaching bacteria L. ferriphilumYSK adsorption behavior of pyrite surface have a certain influence, the optimal growth temperature adsorption highest. The studies show that the surface properties of the bacteria and minerals different matrix the Growth L.ferriphilumYSK surface properties occur certain changes, cultured bacterial pyrite cultured bacteria than ferrous large contact angle, hydrophobicity, but they are The surface properties of pyrite were similar. TEM observation of the surface protein of bioleaching bacteria showed that the solid matrix (pyrite) than the growth of bacteria in the soluble ferrous ions in the growth of the bacterial surface protein, which also contributed to the hydrophobic solid substrate cultivation of bacteria The big reason. Adsorption of bacteria of the isoelectric point of the pyrite direction offset towards the isoelectric point of bacteria. The metabolism of the bacteria in the leaching process makes the iron pyrite formed on the surface of Fe2O3, FeOOH Fe2 (SO4) 3 hydrophilic substances such as lead pyrite surface hydrophilic increase. The atomic force microscope tests show that L. ferriphilum bacteria on pyrite corrosion ability.

Study on Attachment of Leptospirllum Ferriphilum YSK Grown on Different Energy Sources to Pyrite Surface and Surface Properties

Abstract

Related Dissertations