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Dynamic Changes of Cardiovascular Function of Non-Lethal Mechanical Traumatic Rats in the Episode of Clinical Monitor

Author: WeiXiaoHong
Tutor: LiuHuiRong
School: Shanxi Medical
Course: Physiology
Keywords: Non-fatal Mechanical trauma Heart function Vascular function
CLC: R54
Type: Master's thesis
Year: 2010
Downloads: 22
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Abstract

Research background With the the rapid development of of modern industrial, the construction industry, the traffic is the transport industry,, etc., the mechanical trauma the incidence of increased year by year, has become the leading cause of death of crowd in the the following the age of 45. In recent years, due to the the the step-by-step improvement of of the on-site First aid measures, the mechanical trauma a direct result of of the tissue injury has been has been effectively controlled. The organ function of but the secondary to-sexual organizations failure, especially is heart and other life organ failure, and gradually become the the threat the the the an important the pathophysiology of of of of the the life of the injured change the. There are clinical reports pointed out that, generally in good condition in the observed by the part of the non---fatal mechanical trauma patients in the the of Clinical Emergency observation period within 24 hours of, but in the the the a period of time after the the post-discharge will be secondary the cardiac function to reduce the or myocardial infarction. These Study on is prompted to mechanical the trauma for, further after the exist the a kind of indirect types of the secondary to-sexual circulatory system features on obstacles to, but the the such obstacles is the stems from to the heart itself still blood vessels, or the to both for of both does not clearly. Research purposes the use of In this study, non--fatal mechanical trauma rat model of, the of Clinical Emergency Call heart and blood vessels of the observation period the the dynamic changes of of the function in the observe the post-traumatic, in in order to to looking for possible are associated with secondary the circulatory system function obstacles-related indicators. Research Method 1. The Preparation of of the non--fatal mechanical trauma model selection of 80 male Sprague-adult SD rats were (2-3 months), were randomly divided into 2 groups: pseudo-trauma group and post-traumatic group. After the (1) Pseudo - trauma now Group: randomly selected 40 only normal of in-adult rats intraperitoneal injection of on Narcotic rat, the will rats fixed the within a diameter for the the the Noble-Collip trauma MIRIAM in the the of of 30cm as, In to the of the rotational speed of 40 transfected (s) / stars in at turn the 5 minutes by, Great harbourage for rats along with the trauma instrument turn the them without drop them by the a height. Is divided into the following several groups of: ① of no later than 0 hour group after the in the pseudo-trauma (0h): n = 8; of no later than 3 hour group after the in the ② pseudo-trauma (3h): n = group on the 8 ③ pseudo-trauma 6 hours after the (6h): n = 8; ④ of no later than 12 hour group after the in the pseudo-trauma (12h): n = 8; 24-hour group in the ⑤ pseudo-post-traumatic (24h): n = 8 (2) the trauma group: that with 10% chloral hydrate (0.3ml/100g) intraperitoneal injection of experience is anesthesia and large after of Rat, the rats were is which placed in that the diameter for the the the Noble-Collip trauma MIRIAM in the the of of 30cm as, In the 40 transfected (s) / the rotational speed of of the stars in at turn the 5 minutes by to enable the animals to in the the transfected the process of in the a to drop by the a height caused by the mechanical sexual trauma. Trauma group but also were randomly divided into the the following several groups of: ① 0 hour group in the post-traumatic (0h): n = 8; of no later than 3 hour group after the in the ② trauma (3h): n = 8; the group of 6 hours after the in ③ trauma (6h): n = 8; ④ trauma 12 hours after group (12h): n = 8; of no later than 24 hour group after the in the ⑤ trauma (24h): n = 8. 2. with a the MS2000 biological signal recording system monitoring rats in each group heart left ventricular systolic pressure (Left Ventricular Systolic Pressure, LVSP), left ventricular diastolic blood pressure (Left Ventricular Diastolic Pressure, LVDP), left ventricular end diastolic pressure (Left Ventricular End-diastolic Pressure, LVEDP), left ventricular pressure rise / fall maximum rate of change (dp / dtmax and -dp/dtmax), etc. cardiac hemodynamics indicators. 3. The use of the away from the the the the diastolic function of of the rats in each group thoracic aorta of the body-vascular ring technology detection. 4. ELISA method detection serum superoxide-sensitivity C-reaction the protein (hs-CRP) and the myeloperoxidase (MPO) the level of. A [Study on results 1]. A from non-lethal sexual mechanical trauma model create success will a 40-only trauma Groups rats were placed in to after the for in the the rotational speed of in order to 40 transfected (s) / stars have of the in the trauma MIRIAM at turn the 5 minutes by, there are The for 2 But because the Death to the brain surprises trauma For at, the the the remaining were not appears hemorrhagic shock. The pseudo-trauma group and the trauma group rats in the the the at different time points of the within 24h after in the trauma of its MABP gt; 75mmHg, and between the two groups at each time point were not statistically significant (P gt; 0.05). In addition, the the the rats of the trauma group did not caused by the directly the heart of damage, such as pericardial bleeding, cardiac contusion, and and so on on, also no ECG abnormalities. Approximately 2-3h, its behavior, diet and and other activities in the the regained consciousness after of the rats returned to normal, 24h survival rate for the 100%. 2. The dynamically change the of the cardiac function in rats with in the mechanical post-traumatic of non--fatal 2.1 the function of the in Rat Hearts with Myocardial contraction and in the post-traumatic of mechanical sexual did not happen to change the ± dp / dtmax, LVSP, respectively, on behalf of the left ventricular pressure Rise / Fall maximum rate of change and left ventricular systolic blood pressure , they can be reflect the the the heart systolic function. The results of this study display, post-traumatic 0h, 3h, 6h, 12h, the trauma group rats in the 24h when ± dp / dtmax and the between LVSP and the pseudo-trauma group rats compared to the were not statistically different (P gt; 0.05, Figure 1, FIG.. 2, the FIG.-. 3). 2.2 mechanical sexual trauma at 3h after appear in obvious myocardial diastolic dysfunction LVDP, LVEDP, respectively, on behalf of the pressure at the end of at the diastolic of the left ventricular diastolic blood pressure and left ventricular. By the has been observed, the the mechanical post-traumatic of the non--fatal, the the myocardial diastolic function for trauma rats be reduced. In the when 3h of the post-traumatic, to trauma group rats LVDP and LVEDP were elevated, reached the peak (6.90 ± 0.27 mmHg vs. Pseudo-trauma group 1.04 ± 0.26mmHg, (P <0.01); 27.02 ± 2.03 mmHg vs.. Pseudo-trauma group 6.90 ± 0.73 mmHg, p <0.01). But in the the the the when 24h of the post-traumatic, the rat LVDP of trauma group and LVEDP are returned to normal. (Figure 4, Figure 5) 3. The mechanical post-traumatic of the non--fatal endothelial dysfunction of the rat vascular will be trauma group the thoracic aorta in rats of the and pseudo-trauma group taken out, to carried out the away from the the body vascular ring function Determination of, The results showed that, nonfatal mechanical post-traumatic the-dependent diastolic function of the 6h thoracic aorta in rats vascular endothelial decreased significantly, the blood vessels's largest-diastolic the degree of 97.88% ± 9.52% of the by the pseudo-trauma group reduce the to the trauma group of the 50.50% ± 2.44%, the Compared the two there was significant difference in (P lt; Are 0.01, FIG.-6, Are FIG.. 7, Are in FIG.. 8). 4. Serum ultra--sensitivity C-in the post-traumatic the the dynamic changes of of the serum superoxide-sensitive C-reactive protein and myeloperoxidase a 4.1 of mechanical post-traumatic reactive protein levels significantly elevated serum ultra--sensitized Channels C reactive protein levels is to decide on the tissue injury in a sensitive indicator of, not only can reflect the activity of inflammation, has also been considered to be cardiovascular disease in monitoring condition and prognosis of judgment an important indicator of. We in order to the non--fatal mechanical trauma rat model of for the the observation object, to carried out the detection of this indicator. The results said, the Compared with the pseudo-trauma group rats (4312.97 ± 169.99ng/ml), 24 hours after the in the trauma trauma group serum ultra-in rats-sensitivity C-reactive protein levels as high as (8306.22. ± 425.64ng/ml), Compared the two There are statistically significant (P lt; 0.01, Figure 9) the enzyme levels of the serum myeloperoxidase after the the 4.2 mechanical sexual trauma no change in myeloperoxidase can be used as the the a to predict the future have occurred cardiovascular events before indicators, the level of MPO With the the serum elevated, incidence of cardiovascular events significantly increased. The results of this Experimental Study of show that the, compared to with the pseudo-trauma group rats, post-traumatic 0h, 3h, 6h, 12h, level of MPO was in the in the the rat serum in the 24h when There was no significant changes in (P gt; 0.05, Figure 10). Conclusion non-within 24 hours after the of the the mechanical trauma of the lethal, the the a diastolic of features on rat heart. A transient reduce the, the-dependent diastolic function of the thoracic aortic endothelial reduce the, may be related to to the is secondary to heart function was reduced in the post-traumatic for the relevant; while the some of the key protein in the in serum likely to become the on the-clinical non-fatal the the a predictor of of the in patients with mechanical trauma.

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