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Objective: To observe viral myocarditis (Vital Myocarditis, VMC) mice myocardial osteopontin expression (osteopontin, OPN), to explore the significance of OPN expression in viral myocarditis. Methods: Coxsackie virus B3 (Coxsackie virus B3, CVB3) infection of BALB / C mice (n = 32) to establish an animal model of viral myocarditis, and at the same time, a normal group (n = 32), take the myocardial tissue biopsy, HE and Masson staining, and calculate the pathological scores and myocardial collagen fiber volume fraction (CVF), RT-PCR and ELISA assay OPN expression by RT-PCR to detect the content of type I collagen (Col I) and correlation analysis. Results: 1. Coxsackievirus B3 can be successfully established BALB / C mice with viral myocarditis animal models. 2 histopathological changes: the VMC early myocardial necrosis, inflammatory reaction, the convalescent inflammatory cells gradually disappear, significant proliferation of collagen. (3) the myocardial pathological Points: virus group at each time point myocardial pathological scores was significantly higher (P lt; 0.05), seven days increased the most obvious, gradually decreased after 14 days, 28 days increased the amplitude minimum. OPN mRNA expression of 4.RT-PCR detection of myocardial tissue: the control group and the group of viruses mouse myocardium have OPN mRNA expression, the virus of mice myocardial tissue OPN mRNA expression is highest in the first 7 days, 14 days after the expression began to decline day 28 expression is low, but higher than the content in myocardial tissue in mice (P lt; 0.05) OPN expression 5.Elisa assay myocardial tissue: virus mice OPN concentration highest in the first 7 days, 14 days, gradually decreased, 28 days to a minimum, but were higher than those in the control group (P gt; 0.05) 6 the myocardial fiber volume fraction (CVF) results show that: virus group on day 7 to 14 days CVF with the control group was not significantly different (P gt; 0.05); virus 21 days, 28 days CVF with the control group virus group, 7 days, 14 days were significantly different (P lt; 0.05) 7.RT-PCR detection of myocardial tissue of type I collagen mRNA expression (Col I mRNA): virus group on day 7, the 14th day of myocardial Col I expression is weak, compared with the same period in the control group, no significant difference (P GT; 0.05), 14 days after the enhancement, 28 days maximum, 21 days, 28 days Col I mRNA / GAPDH optical density value of the control group, 7 days, 14 days more significant difference (P lt; 0.05). Correlation analysis: OPN mRNA expression and myocardial pathology integral to do correlation analysis, R2 = 0.8877, both showed a positive correlation; correlation analysis for Col I mRNA and CVF, R2 = .9411, both showed a positive correlation; myocardial Col I mRNA and OPN mRNA expression correlation analysis, R2 = 0.3322, no correlation between the two. Conclusion: OPN may be involved in the the VMC acute phase inflammatory response, and may promote collagen synthesis during the recovery period.
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