Dissertation > Excellent graduate degree dissertation topics show

The Expression and Characterization of Mycobacterium Tuberculosis DNA Repair Gene fpg

Author: HuJin
Tutor: XieJianPing
School: Southwestern University
Course: Microbial and Biochemical Pharmacy
Keywords: DNA repair Base Excision Repair formamidopyrimidine-DNA glycosylase Mycobacterium tuberculosis
CLC: R378
Type: Master's thesis
Year: 2010
Downloads: 28
Quote: 1
Read: Download Dissertation


TB remains a major global threat to human health. There are almost 17-20 million people infected with MTB (Mycobacterium tuberculosis) worldwidely, and 3 million new patients annually. China ranks top after India as heavy-burden TB incidence. Latent MTB infection directly led to a long course of TB treatment, poor efficacy, and easy to relapse after chemotherapy, because of course time turns so long, most people can not afford the necessary medical costs, patients are not fully chemotherapy, and this is the reasons of high relapse rate and drug resistance of TB cases increased, also that is why TB is hard to cure.The maintenance of genome stability is the crux for organism survival. Base excision repair (BER) is a major DNA repair pathway. This is particularly important for Mycobacterium tuberculosis and other intracellular pathogens, fpg is the key enzyme in base excision repair. Mycobaterium genome comparison revealed that pathogenic M.tuberculosis harbor more genes involved in the BER. This might implicate that BER is closely related to the intracellular survival and pathogenesis of this bug. This justifies the BER pathway and crucial enzymes as promising targets for novel antibiotics.fpg of M. tuberculosis H37Rv was cloned into pET32a(+) and expressed in E.coli BL21(DE3) with the induction of IPTQ and subjected to Ni2+ affinity chromatograph. The comparison of the control and fpg transformed host demonstrated that the fpg gene plays a role in the peroxide hydrogen and UV irradiation resistance.

Related Dissertations

  1. Study on the Correlation of murA Gene and Mycobacterial Growth,Q78
  2. Expression and Functional Analysis of M. Tuberculosis WecA Membrane Protein,R378
  3. Mycobacterium tuberculosis Rv3717 gene cloning , expression and functional identification,R378
  4. Mycobacterium tuberculosis Rv1096 expression and functional identification,R378
  5. Extraction of Lipoarabinomannan from Mycobacterium Tuberculosis and Detection of LAM-IgG in Serum for the Diagnosis of Tuberculosis,R521
  6. Screening of Specific Antibody Binding Peptides and Applied Research in Serology Detection of Mycobacterium Tuberculosis,R52
  7. Cloning Expression and Biochemical Characterization of Uracil-dna Glycosylase from P. Furiosus,Q78
  8. Radioprotective Effects and Its Mechanism of Genistein on X- Ray Induced DNA Damage on Human Liver L-02 Cells,R965
  9. Genetic Variants of APE1 Gene Contribute to Susceptibility to Cervical Cancer and Their Mechanisms,R737.33
  10. Occupational 1,3 - butadiene workers DNA damage and repair markers of,R131
  11. Study on the Association of XRCC1 and SMAD4 Polymorphisms and the Susceptibility of Gastric Cancer,R735.2
  12. To Investigate the Expression of HnRNP A2/B1 in NSCLC and the Role of HnRNP A2/B1 in the Pathogenesis of NSCLC,R734.2
  13. Analysis of Cell Cycle’s Correlation of Phosphorylation of H2AX,R346
  14. Xeroderma Pigmentosum D Gene Regulates the Expression of p52 and p21 in Hepatoma,R735.7
  15. Generation of Reference Standards and Development of a Novel Method for Detection of Culture Filtrate Protein 10 of Mycobacterium Tuberculosis,R378
  16. Effects of ATM Gene Silencing by miRNA on DNA Damage and Repair of Hepatoma Cells after Ionizing Radiation,Q691
  17. Association between DNA Repair Gene Polymorphisms and Environmental Factors and Hp-associated Gastric Cancer and Duodenal Ulcer,R735.2
  18. Relationship Between RAD51 5’-UTR G135C Polymorphisms and Therapy Response to Platinum-Based Chemotherpy of Advanced Non-Small Cell Lung Cancer Patients,R734.2
  19. Esophageal cancer cells by X-ray irradiation of DNA repair gene expression analysis,R735.1
  20. The Relationship between Polymorphism of Gene XPD 751 and Response to Oxaliplatin-based Chemotherapy in Advanced Gastric Cancer,R735.2

CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic bacteria
© 2012 www.DissertationTopic.Net  Mobile