Dissertation > Excellent graduate degree dissertation topics show

Extraction and Separation of Paeoniflorin & Albiflorin from Radix Paeoniae Rubra

Author: LiZuo
Tutor: WangShaoYan
School: Liaoning University of Science and Technology
Course: Applied Chemistry
Keywords: Paeoniflorin Albiflorin SMBC Macroporous resin
CLC: R286
Type: Master's thesis
Year: 2012
Downloads: 195
Quote: 0
Read: Download Dissertation

Abstract


Radix Paeoniae Rubra is one kind of Chinese traditional medicine. Peaoniflorin and Albiflorin are important active components in Radix Paeoniae Rubra, each of which has unique pharmacological value. In this paper, the crude extract was firstly obtained from Radix Paeoniae Rubra by solvent extraction. The extract conditions including liquid-solid ratio, extraction solvent, extraction time and extraction temperature were optimized by orthogonal design, uniform design and spherical symmetric design, respectively. Then, the mixture of Paeoniflorin and albiflorin was separated by macroporous resin from the crude extract, the separation results were compared using two types of resin, AB-8 and D101. The eluent and elution volume were also selected. Paeoniflorin and albiflorin were further purified by simulated moving bed chromatography(SMBC). Switching time, the mobile phase, elution flow rate, and other parameters were selected, and the operation stability of SMB was investigated. Experimental results showed that the optimized extracting conditions were as follows: methanol concentration 90%, liquid-solid ratio 10～12, extraction time 180min, extraction temperature 40℃. The gradient elution was used in the separation of the two components by D101 resin, elution program was deionized water (2170mL) -20% methanol in water (1250mL) -60% methanol in water (980mL) - Pure methanol (700mL). The mixture of Paeoniflorin and Albiflorin was finally separated by simulated moving bed, and the main process parameters were as follows: four chromatographic column (ID×L = 1.0×20.0cm), stationary phase was 20μm ODS packing; mobile phase was methanol: water =3:7(V/V); eluent flow rate 2.0mL/min; purging flow rate 4.0 mL/min; injection flow rate 0.1mL/min; feed concentration 0.1g/mL; switching time 25 min; operating at room temperature. Under the above conditions, the mixture was separated by SMB, and more than 92% and 90% content of Paeoniflorin and Albiflorin were obtained, respectively. The SMB separation process was stable.

Related Dissertations

  1. Studies Preparation of Procyanidines from Grape Seeds and Mycotoxins Determination,TQ461
  2. Extraction Condition of Paeoniflorin from Paeonia Lactiflora,R284.2
  3. Research on the Effects and Potassium Ion Channels Mechanism of Paeoniflorin in Regulating the Motility of Oddi′s Sphincter Smooth Muscle Cells,R285
  4. Study on the Preparation Technology and Quality Standard of Tongmai Drop Pills,TQ461
  5. To Study the Techniques for Purification of Nature Products by High-speed Counter-current Chromatography(Ⅱ),R284
  6. Adsorption of 4-Nitrobenzonitrile and 4-Nitroanisole on NDA-150 Resin,X787
  7. Adsorption Performance of CO2 on Different Spherical Porous Materials,X51
  8. Study on Extraction, Purification and Antioxidant Activities of Total Flavonoids from Peanut Shell,TQ461
  9. Studies on Extraction, Separation and Purification of Aloe-sin,R284
  10. Special anti-TB drug resistance degree Capsule Preparation and Quality Standard,TQ463
  11. Study on Preparation and Antioxidant Activities of Procyanidins from the Seedpod of Nelumbo Nucifera Gaertn,R285
  12. Study on the Extraction, Purification and Antioxidant Activity of Polyphenols from Seed of Camellia Oliefer,TQ914.1
  13. Paeoniflorin Suppreses Nf-κb Activation Through Modulation of Iκbα and Enhances the Growth Inhibition and Apoptosis of Human Gastric Carcinoma Cells,R735.2
  14. Effect of Paeoniflorin on Drug Resistance of Human Gastric Cancer SGC7901/VCR Cells and Its Mechanism,R735.2
  15. Study on the Preparation Process and Quality Standard of Invigorating Spleen and Resolving Dampness Grains,R283
  16. Studies on the Optimization of Fermentation Conditions for Zuelacmycin-producing Strain GAO-1-GR-2 and Distraction Characteristics for Bioactive Substances,TQ920.6
  17. Preparationing Diosgenin from Dioscorea Zingiberensis,TQ464.3
  18. Study on Extraction and Separation of Tea Polyphenols Form Green Tea,TQ463
  19. Effect of Dynamic High Pressure Microfluidization on Extraction, Structure and Antioxidant of Flavonoids from Sweet Phtato Leaves,TQ914.1
  20. Research on the Process of Microwave-assisted Extraction and Separation and Purification of Soy Isoflavones,TQ914.1
  21. Research on the Separation Process of Hydroxysafflor Yellow A,TQ461

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > In drugs
© 2012 www.DissertationTopic.Net  Mobile