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Interactions between IAA and GA3 in Regulation of Etiolated Stem Segment Elongation in Pisum Sativum L.

Author: JiangYuXia
Tutor: XiaKai
School: Nanjing Agricultural College
Course: Botany
Keywords: Auxin GA Stem segments Elongation Interaction
CLC: Q945
Type: Master's thesis
Year: 2006
Downloads: 55
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Abstract


The classic five categories of plant hormones auxin (auxin) and GA (gibberellin, GA) in the regulation of plant growth plays a very important role. However, people began to focus on recent studies the interaction between the two hormones. Many studies have confirmed: auxin can promote the activity of gibberellin synthesis, thereby promoting stem elongation. Ross et pea wild type as the material in the biological activity studies have shown that ghrelin GA 1 plays an important role in the synthesis. Pea terminal buds of auxin can promote LE gene (PsGA3ox1 gene) transcript levels, thereby increasing the GA 20 to GA 1 conversion; while reducing the SLN gene (ie PsGA2ox1 ) transcription levels, reduce GA 20 to non-active GA 29 and GA 1 to non-active GA 8 of conversion. Auxin by promoting GA 1 biosynthesis, while suppressing GA 1 inactivation of elongation of internodes in regulating peas GA 1 content. However, most of these experiments to study the whole plant auxin and gibberellin relationship between stem segments treatment experiment, it only provides for an active GA studied the precursor under conditions of IAA on the level of activity of GA . In this experiment, Etiolated as materials, IAA, GA 3 to study various processes the relationship between the two. 1 IAA and GA 3 can be individually promote Etiolated stem segments elongation growth with 2,10,50,250 μmol · L -1 of IAA and GA < sub> 3 treatment between third 1cm cut into sections, can promote Etiolated elongation of stem segments in which to 250μmol · L -1 of IAA and 50μmol · L -1 of GA 3 role in promoting best elongation control respectively 2.14 times and 2.21 times. 80mg · L -1 TIBA (IAA transport inhibitor) and 7.5mg · L -1 S-3307 (active GA synthesis inhibitor) were inhibited Wandou of seedling elongation of stem segments, the growth of the treated cuttings were respectively 66.3% and only 55.4%. IAA on elongation growth of stem segments focused on promoting the role of pre-treatment, the growth rate reached the highest value in 1h. The GA 3 process focused on the treatment late, the growth rate reached the highest value at 6h. 2 IAA and GA 3 in the regulation of Etiolated stem segments elongation relationship in 2.1 GA 3 can improve Etiolated stem segments on the IAA The sensitivity with GA 3 Pre-treatment stem segments 24h, 24h and then after IAA treatment, stem segments elongation reached 0.90cm, is free of hormone treatment were 2.94 times. Current processing time is between 3-6h on stem segments elongation greater role in promoting. In 4h GA 3 pre-treatment and then treated with IAA, 3h elongation when the stem segments was significantly greater than the amount of hormone-treated controls. This indicates that GA 3 stem segments can improve the sensitivity for IAA and shorter duration of action (3h). Pre-treatment with IAA stem segments, then GA 3 treatment, stem segments elongation than IAA, GA 3 separate treatment elongation, while re- Add to IAA treatment, then can resume even more than a single IAA, GA 3 handle stem segments elongation. This goes also confirmed the above points. 2.2 IAA and GA 3 in Etiolated stem segments elongation indispensable role in 250μmol · L -1 added 7.5 mg of IAA · L -1 S-3307 after, IAA effect can be suppressed by S-3307. Similarly, in 50μmol · L -1 GA 3 add 80 mg · L -1 TIBA after, GA 3 effect was also inhibited by TIBA. And then were added GA 3 and IAA stem segments will recover after elongation growth, suggesting that both in the Etiolated stem segments elongation growth are indispensable. Cut in half on top of the handle stem segments observed, IAA deal cut sections were cut in half curved inward, while GA 3 process is the opposite. Description IAA epidermal stem segments mainly promote cell elongation, while GA 3 may be mainly to promote the internal organization of cell elongation. Right cut for horizontal and vertical slices observed, IAA promote cortical cell elongation and increased cell length and longitudinal section transverse section of the control cell area was 1.21 times and 1.72 times. The GA 3 only promote cortical cell elongation, longitudinal section of the control cell length 1.82 times. Reveals a different role both through the site and the way the regulation of pea stem segments elongation growth. 2.3 IAA and GA 3 right Etiolated stem segments of endogenous GA 1 3 and IAA content of 50μmol · L -1 GA 3 treatment can also promote stem segments endogenous IAA content increases, 24 h when the IAA content of 311.7pmol · (g · FW) -1 , is CK 1.9 times. And 250μmol · L -1 IAA treatment can increase Etiolated stem segments of endogenous GA 1 3 content, 24 h when the GA 1 3 < / sub> content of 652.2 pmol · (g · FW) -1 , is 3.2 times of CK. Endogenous IAA treated GA 1 3 levels greater than GA 3 treatment endogenous IAA levels, indicating possible GA 3 and IAA respective positive to regulate the biosynthesis and other levels but GA 3 to handle the increased IAA levels on Etiolated stem segments Elongation relatively small.

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CLC: > Biological Sciences > Botany > Plant Physiology
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